Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes.
Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes.
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DOI：
10.1007/978-1-0716-0668-1_10
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kühn B
中科院分区：
文献类型：
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作者：
Han L;Mich-Basso J;Kühn B
损伤后心肌不能再生是成年人死亡和发病的主要原因。人类诱导多能干细胞（iPSC）直接分化为心肌细胞为追求基于细胞的心脏再生提供了宝贵的资源。除了临床治疗的潜力外，iPSC技术还可以生成心肌细胞，从而重现患者特定的表型，从而提供了一个强大的体外细胞模型，以了解疾病病理学并指导精准医学。在这里，我们描述了通过非整合仙台病毒系统将人皮肤成纤维细胞和血细胞重编程为iPSCs的方案，以及使用化学成分确定的培养基将iPSCs单层分化为心肌细胞的方案。
Failure to regenerate myocardium after injury is a major cause of mortality and morbidity in adult humans. Direct differentiation of human induced pluripotent stem cells (iPSCs) into cardiomyocytes provides an invaluable resource to pursue cell-based cardiac regeneration. Beyond the potential for clinical therapies, iPSC technology also enables the generation of cardiomyocytes, which recapitulates patient-specific phenotypes, thus presenting a powerful in-vitro cell-based model to understand disease pathology and guide precision medicine. Here we describe protocols for reprogramming human dermal fibroblasts and blood cells into iPSCs by the non-integrative Sendai virus system and for the monolayer differentiation of iPSCs to cardiomyocytes using chemically defined media.