Heterologous Expression of Pteris vittata Phosphate Transporter PvPht1;3 Enhances Arsenic Translocation to and Accumulation in Tobacco Shoots
Heterologous Expression of Pteris vittata Phosphate Transporter PvPht1;3 Enhances Arsenic Translocation to and Accumulation in Tobacco Shoots
复制标题
凤尾蕨磷酸盐转运蛋白 PvPht1;3 的异源表达增强砷在烟草芽中的转运和积累
DOI：
10.1021/acs.est.9b02082
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发表时间：
2019
影响因子：
11.4
通讯作者：
Lena Q. Ma
中科院分区：
文献类型：
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作者：
Yue Cao;Huayuan Feng;Dan Sun;Guohua Xu;Bala Rathinasabapathi;Yanshan Chen;Lena Q. Ma
砷超富集植物蜈蚣蕨具有富集砷的能力，已被应用于砷污染土壤的植物修复。本文克隆了PvPht 1;3基因的全长编码序列，并研究了其在酵母和植物积累砷中的作用。PvPht 1;3与酵母磷吸收突变株互补，对AsV的亲和性和转运能力均比PvPht 1;2强。在转基因烟草中，PvPht 1; 3促进了AsV的吸收和转运，增加了水培和土壤条件下地上部As的积累。通过qRT-PCR的表达模式的基础上，PvPht 1;3强烈诱导磷缺乏，但不作为暴露。为了进一步了解该基因的表达模式，我们在拟南芥和大豆中构建了由PvPht 1;3启动子驱动的GUS报告基因转基因植株。GUS染色结果表明，PvPht 1;3主要在中柱细胞中表达，可能参与了P/A3的转座。综上所述，PvPht 1;3是一种高亲和力的AsV转运蛋白，可能是实现As高效转运的主要途径。我们的研究结果表明，表达PvPht 1;3增强了As在植物中的转运和积累，从而改善了As污染土壤的植物修复。
Arsenic-hyperaccumulator Pteris vittata isefficient in As accumulation and has been used inphytoremediation of As-contaminated soils. Arsenate (AsV)is the predominant As species in aerobic soils and is taken upby plants via phosphate transporters (Pht) including P. vittata.In this work, we cloned the PvPht1;3 full length codingsequence from P. vittata and investigated its role in Asaccumulation by yeast and plants. PvPht1;3 complemented ayeast P uptake mutant strain and showed a stronger affinityand transport capacity to AsV than PvPht1;2. In transgenictobacco, PvPht1;3 enhanced AsV absorption and translocation, increasing As accumulation in the shoots underboth hydroponic and soil experiments. On the basis of the expression patterns via qRT-PCR, PvPht1;3 was strongly induced byP deficiency but not As exposure. To further understand its expression pattern, transgenic Arabidopsis thaliana and soybeanexpressing the GUS reporter gene, driven by PvPht1;3 promoter, were produced. The GUS staining showed that the reportergene was mainly expressed in the stele cells, indicating that PvPht1;3 was expressed in stele cells and was likely involved in P/Astranslocation. Taken together, the data suggested that PvPht1;3 was a high-affinity AsV transporter and was probablyresponsible for efficient As translocation in P. vittata. Our results suggest that expressing PvPht1;3 enhances As translocationand accumulation in plants, thereby improving phytoremediation of As-contaminated soils.