[A preliminary study on the identification and distribution of epidermal stem cells in different degrees of burn wounds in scalded rats].
[A preliminary study on the identification and distribution of epidermal stem cells in different degrees of burn wounds in scalded rats].
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发表时间：
2003-12
影响因子：
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通讯作者：
Julin Xie;Tian-zeng Li;S. Qi;H. Bian;Jian-ding Cheng;Yingbin Xu;Hui-zhen Liang
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作者：
Julin Xie;Tian-zeng Li;S. Qi;H. Bian;Jian-ding Cheng;Yingbin Xu;Hui-zhen Liang
目的探讨表皮干细胞（ESCs）在烫伤大鼠不同程度创面中的分布。方法SD大鼠32只。在大鼠皮肤上形成一度（I）、浅（浅II）和深部分厚度（深II）以及全厚度烧伤伤口（III）。在烧伤后24小时（PBH）从所有伤口收获烧伤伤口样品并处理成组织切片。组织切片行免疫组织化学染色。以整合素α 2 β 1和角蛋白10（K10）为第一抗体，观察不同程度烧伤创面ESCs的表达和分布。结果Ⅰ度烧伤创面K10阳性细胞主要分布于棘层、颗粒层和透明层，在残留的基底层和皮肤附件中有大量整合素α 2 β 1阳性细胞浅Ⅱ度烧伤创面的毛囊深Ⅱ度烧伤创面真皮深层皮肤附件中整合素α 2 β 1阳性细胞较少。最后，整合素α 2 β 1阳性细胞稀疏地发现在III度烧伤创面。结论ESCs在烧伤创面的分布与创面深度密切相关。残余的ESCs可能是烧伤创面再生和再上皮化的来源。
OBJECTIVE To investigate the distribution of epidermal stem cells (ESCs) in different degrees of burn wounds in scalded rats. METHODS Thirty-two Sprague-Dawley (SD) rats were employed in the study. First degree (I), shallow (shallow II) and deep partial thickness (deep II) and full thickness burn wounds (III) were created on the rat skin. Burn wound samples were harvested at 24 postburn hours (PBHs) from all the wounds and were processed to tissue slices. The tissue slices were stained by immunohistochemistry technique. The expression and distribution of ESCs in different degrees of burn wounds were observed with integrins alpha 2 beta 1 and keratin 10 (K10) as first antibodies. RESULTS K10 positive cells were found to distribute in the strata spinosum, granulosum and lucidum in the first degree burn wound (I) with large amounts of integrins alpha 2 beta 1 positive cells in the residual basal layer and skin appendages (hair follicles) in shallow partial thickness burn wound (shallow II degree), and there were less integrins alpha 2 beta 1 positive cells in the remaining skin appendages in deep dermis in deep partial thickness burn wound (deep II degree). Finally, integrins alpha 2 beta 1 positive cells were sparsely found in the III degree burn wound. CONCLUSION The distribution of ESCs in burn wounds was closely related to the depth of burn wound. The residual ESCs might be the origin of burn wound regeneration and reepithelization.