Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer
Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer
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DOI：
10.1111/j.1440-1746.2008.05490.x
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发表时间：
2008-08-01
影响因子：
4.1
通讯作者：
Ramakrishna, Balakrishnan S.
中科院分区：
文献类型：
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作者：
Balamurugan, Ramadass;Rajendiran, Ethendhar;Ramakrishna, Balakrishnan S.
背景和目的：肠道产生的细菌代谢产物可能与结直肠癌的发生有关。丁酸盐可以预防癌症，而硫化氢和氧自由基对上皮细胞有毒性。对20例结直肠癌患者、9例上消化道肿瘤患者和17例健康志愿者粪便中的直肠杆菌、普鲁斯尼茨杆菌(均为丁酸产生菌)、硫酸盐还原菌(硫酸盐还原菌)和粪肠球菌(产生胞外超氧化物)进行了定量检测。利用针对16S rDNA的实时定量聚合酶链式反应对上述细菌种或属进行定量，并与所有细菌之间保守的通用序列的扩增相关。结果：与健康志愿者相比，结直肠癌患者的大肠埃希菌和普氏福氏杆菌的水平下降了近4倍(P分别为0.0088和0.0028)。三组间的脱硫弧菌水平无显著差异。结直肠癌患者粪肠球菌数量明显高于健康志愿者(P=0.0294)。结论：结肠癌患者粪便中丁酸产生菌减少，粪肠球菌增多。结肠细菌种群的这些变化可能会潜在地导致上皮细胞损伤和周转增加，并可能是导致结肠癌的一个因素。
Background and Aim: Bacterial metabolites produced in the bowel are potentially related to the genesis of colorectal cancer. Butyrate is protective against cancer, whereas hydrogen sulfide and oxygen free radicals can be toxic to the epithelium. The present study was designed to quantitate Eubacterium rectale, Faecalibacterium prausnitzii (both butyrate-producing bacteria), Desulfovibrio (sulfate-reducing bacteria), and Enterococcus faecalis (that produces extracellular superoxide) in the feces of patients with colorectal cancer.Methods: DNA was extracted from feces of 20 patients with colorectal cancer, nine patients with upper gastrointestinal cancer and 17 healthy volunteers. Real-time polymerase chain reaction using primers aimed at 16S rDNA was used to quantitate the above bacterial species or genus, and this was expressed relative to amplification of universal sequences conserved among all bacteria.Results: Levels of E. rectale and F. prausnitzii were decreased approximately fourfold (P = 0.0088 and 0.0028, respectively) in colorectal cancer patients compared to healthy control volunteers. Levels of Desulfovibrio were not significantly different between the three groups. E. faecalis populations were significantly higher in colorectal cancer patients compared to healthy volunteers (P = 0.0294).Conclusions: Butyrate producers were decreased and E. faecalis increased in the feces of colon cancer patients. These shifts in the colonic bacterial population could potentially lead to epithelial cell damage and increased turnover and may be a factor leading to colon cancer.