IgLON cell adhesion molecule Kilon is a crucial modulator for synapse number in hippocampal neurons
IgLON cell adhesion molecule Kilon is a crucial modulator for synapse number in hippocampal neurons
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DOI：
10.1016/j.brainres.2008.05.069
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发表时间：
2008-08-11
期刊：
影响因子：
2.9
通讯作者：
Miyata, Seiji
中科院分区：
文献类型：
--
作者：
Hashimoto, Takashi;Yamada, Mayumi;Miyata, Seiji
Kilon是IgLON家族的成员，属于细胞粘附分子的免疫球蛋白超家族。本研究以培养的海马神经元为材料，探讨Kilon表达的时空变化及其对突触数目的调节作用。Kilon在培养早期主要定位于轴突和突触前终末，而在培养晚期主要定位于成熟神经元的树突状突触后棘。在早期培养阶段，Kilon被洗涤剂处理溶解，而在成熟神经元中，它抵抗洗涤剂的提取。过度表达Kilon基因的过表达在早期培养阶段减少了树突状突触的数量，而在晚期培养阶段增加了树突状突触的数量。这些结果表明，在神经元培养发育过程中，Kilon对树突状突触数量的调节功能的改变伴随着其定位和洗涤剂溶解度的变化。(c)2008 Elsevier B.V.保留所有权利。
Kilon is a member of the IgLON family belonging to the immunoglobulin superfamily of cell adhesion molecules. In the present study, we investigated temporal and spatial changes of Kilon expression and its modulatory functions for synapse number using hippocampal cultured neurons. Kilon was observed to localize chiefly at axons and presynaptic terminals at early culture stage, however, it was seen mainly at dendritic postsynaptic spine of mature neurons at late culture stages. Kilon was solubilized with detergent treatment at early culture stages, while it resisted to extraction of the detergent in mature neurons. The overexpression. of Kilon gene using a plasmid vector decreased the number of dendritic synapses at early culture stages, whereas the overexpression increased the number of dendritic synapses at late culture. These results demonstrate the alteration of modulatory function of Kilon for the number of dendritic synapses concomitant with changes in its localization and detergent solubility during neuronal culture development. (c) 2008 Elsevier B.V. All rights reserved.