Rhythms, synchrony and electrical coupling in the Locus coeruleus
Rhythms, synchrony and electrical coupling in the Locus coeruleus
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DOI：
10.1016/j.resp.2004.07.018
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发表时间：
2004-11-15
影响因子：
2.3
通讯作者：
Scheid, P
中科院分区：
文献类型：
--
作者：
Ballantyne, D;Andrzejewski, M;Scheid, P
神经元的电耦合被认为促进同步活动。然而，在某些系统中，它也可能是维持内源性节律活动的必要条件。在整块分离的脑干脊髓的新生大鼠同时全细胞记录从对LC神经元（n = 47对）披露的大部分强烈同步活动，可以采取的形式紧张性尖峰或相位爆发。同时从LC神经元和胶质细胞的全细胞记录也发现同步波的17对去极化。这种同步性部分是由于化学相突触输入，部分是由于机制，这是不依赖于化学突触。差距连接解偶联剂甘珀酸抑制LC神经元的非突触节律活动，但不抑制这些神经元的非突触相突触输入或其兴奋性反应增加CO2。我们给出了LC神经元之间存在电流通路的初步直接证据，该电流通路被甘珀酸抑制。在LC核内，可能涉及神经元-神经胶质以及神经元-神经元相互作用的对羧甲恶唑啉敏感的电耦合可能不仅是同步化所需的，而且也是维持节律所需的。(C)2004 Elsevier B. V.保留所有权利。
Electrical coupling of neurones is believed to promote synchronized activity. It may, however, also be a requirement for the maintenance of endogenous rhythmic activity in some systems. In en bloc isolated brainstem-spinal cord of the neonatal rat simultaneous whole cell recordings from pairs of LC neurones (n = 47 pairs) disclosed for the most part strongly synchronized activity which could take the form of tonic spiking or phasic bursts. Simultaneous whole cell recording from LC neurones and glia also revealed synchronized waves of depolarization in 7 of 17 pairs. This synchrony was partly due to respiratory-phased synaptic input and partly due to mechanisms, which were not dependent on chemical synapses. The gap junction uncoupler carbenoxolone suppressed non-synaptic rhythmic activity in LC neurones, but did not suppress either respiratory-phased synaptic input to these neurones or their excitatory response to increased CO2. We give preliminary direct evidence for the existence of a current pathway between LC neurones, which is inhibited by carbenoxolone. Within the LC nucleus carbenoxolone-sensitive electrical coupling, which may involve neurone-glia as well as neurone-neurone interactions, may be required not just for synchronization, but also for the maintenance of rhythm. (C) 2004 Elsevier B.V. All rights reserved.