Expression of HpaGXooc Protein in Bacillus subtilis and its Biological Functions
Expression of HpaGXooc Protein in Bacillus subtilis and its Biological Functions
复制标题
HpaGXooc蛋白在枯草芽孢杆菌中的表达及其生物学功能
DOI：
10.4014/jmb.0802.154
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Gao, Xuewen
中科院分区：
文献类型：
--
作者：
Wu Huijun;Wang, Shuai;Gao, Xuewen
HpaG(Xooc), from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the HpaG(Xooc) protein in Bacillus subtilis, we constructed a recombinant expression vector pM43HF with stronger promoter P43 and signal peptide element nprB. The SDS-PAGE and Western blot analysis demonstrated the expression of the protein HpaG(Xooc) in B. subtilis. The ELISA analysis determined the optimum condition for HpaG(Xooc) expression in B. subtilis WBHF. The biological function analysis indicated that the protein HpaG(Xooc) from B. subtilis WBHF elicits hypersensitive response (HR) and enhances the growth of tobacco. The results of RT-PCR analysis revealed that HpaG(Xooc) induces expression of the pathogenesis-related genes PR-1a and PR-1b in plant defense response.
