Identification of 24 new microsatellite loci in the sweat bee Lasioglossum malachurum (Hymenoptera: Halictidae).
Identification of 24 new microsatellite loci in the sweat bee Lasioglossum malachurum (Hymenoptera: Halictidae).
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DOI：
10.1186/s13104-017-3089-4
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发表时间：
2017-12-19
影响因子：
1.8
通讯作者：
Field J
中科院分区：
文献类型：
--
作者：
Parsons PJ;Couchoux C;Horsburgh GJ;Dawson DA;Field J
本文的目的是确定古北界汗蜂马拉舌毛舌蜂的高度多态性微卫星位点。汗蜂（Hyattera：海象科（Halictidae）是分布广泛的传粉者，表现出异常广泛的社会行为，从非社会性，其中每个雌性单独筑巢，到真正的社会性，其中一个单一的女王繁殖，而其他成员的殖民地帮助抚养她的后代。因此，它们代表了理解社会进化的优秀模型。成功优化了24个新的微卫星位点。当在23-40个不相关的女性中扩增时，每个位点的等位基因数范围为3至17，观察到的杂合性为0.45至0.95。只有一个位点显示出显著偏离Hardy-Weinberg平衡的证据。这24个基因座将使研究人员能够更好地了解该物种内的群体关系，这是研究真社会性的一个重要模型。此外，在L. calceatum，这表明这些位点可能是有用的调查生态和进化的汗蜂一般。
The objective here is to identify highly polymorphic microsatellite loci for the Palaearctic sweat bee Lasioglossum malachurum. Sweat bees (Hymenoptera: Halictidae) are widespread pollinators that exhibit an unusually large range of social behaviours from non-social, where each female nests alone, to eusocial, where a single queen reproduces while the other members of the colony help to rear her offspring. They thus represent excellent models for understanding social evolution. 24 new microsatellite loci were successfully optimized. When amplified across 23–40 unrelated females, the number of alleles per locus ranged from 3 to 17 and the observed heterozygosities 0.45 to 0.95. Only one locus showed evidence of significant deviation from Hardy–Weinberg equilibrium. No evidence of linkage disequilibrium was found. These 24 loci will enable researchers to gain greater understanding of colony relationships within this species, an important model for the study of eusociality. Furthermore, 22 of the same loci were also successfully amplified in L. calceatum, suggesting that these loci may be useful for investigating the ecology and evolution of sweat bees in general.