The Fate of Transplanted Periodontal Ligament Stem Cells in Surgically Created Periodontal Defects in Rats
The Fate of Transplanted Periodontal Ligament Stem Cells in Surgically Created Periodontal Defects in Rats
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DOI：
10.3390/ijms20010192
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发表时间：
2019-01-01
影响因子：
5.6
通讯作者：
Morita, Ikuo
中科院分区：
文献类型：
--
作者：
Iwasaki, Kengo;Akazawa, Keiko;Morita, Ikuo
牙周病是一种慢性炎症，导致牙齿支持牙周组织的破坏。我们设计了一种新的方法（细胞转移技术）将细胞转移到支架表面，并报告了该技术用于再生医学的潜力。本研究的目的是检查这种技术在牙周再生和移植细胞的命运的功效。将人牙周膜干细胞（PDLSCs）与脱细胞羊膜复合，移植于大鼠牙周缺损处。通过显微计算机断层扫描和组织学观察来检查组织再生。采用PKH26和人Alu序列PCR检测追踪移植PDLSC的命运。影像学显示，PDLSC移植缺损处的骨比对照组（仅羊膜）多。组织学检查证实PDLSC缺损处牙周组织形成增强。牙骨质、牙周膜和骨的新形成在PDLSC缺损中显著观察到。PKH26标记的PDLSCs在再生牙周组织中的有限区域被发现。人Alu序列检测显示Alu序列水平没有增加，而是下降。这项研究描述了一种新的干细胞移植策略，牙周病使用的细胞转移技术，并提供了新的见解，为细胞为基础的牙周再生。
Periodontal disease is chronic inflammation that leads to the destruction of tooth-supporting periodontal tissues. We devised a novel method (cell transfer technology) to transfer cells onto a scaffold surface and reported the potential of the technique for regenerative medicine. The aim of this study is to examine the efficacy of this technique in periodontal regeneration and the fate of transplanted cells. Human periodontal ligament stem cells (PDLSCs) were transferred to decellularized amniotic membrane and transplanted into periodontal defects in rats. Regeneration of tissues was examined by microcomputed tomography and histological observation. The fate of transplanted PDLSCs was traced using PKH26 and human Alu sequence detection by PCR. Imaging showed more bone in PDLSC-transplanted defects than those in control (amnion only). Histological examination confirmed the enhanced periodontal tissue formation in PDLSC defects. New formation of cementum, periodontal ligament, and bone were prominently observed in PDLSC defects. PKH26-labeled PDLSCs were found at limited areas in regenerated periodontal tissues. Human Alu sequence detection revealed that the level of Alu sequence was not increased, but rather decreased. This study describes a novel stem cell transplantation strategy for periodontal disease using the cell transfer technology and offers new insight for cell-based periodontal regeneration.