Transcriptome and DNA methylome analysis reveal new insights into methyl jasmonate-alleviated chilling injury of peach fruit after cold storage
Transcriptome and DNA methylome analysis reveal new insights into methyl jasmonate-alleviated chilling injury of peach fruit after cold storage
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DOI：
10.1016/j.postharvbio.2022.111915
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发表时间：
2022-03-30
影响因子：
7
通讯作者：
Zhang, Bo
中科院分区：
文献类型：
--
作者：
Duan, Wenyi;Yang, Can;Zhang, Bo
茉莉酸甲酯（MeJA）用于缓解桃等园艺作物采后冷藏引起的冷害。然而，其分子机制在很大程度上仍然未知。在这里，我们处理融化的桃果实（Prunus persica L。巴奇，CV.胡静米露）与不同浓度的MeJA混合，然后在0 ℃下贮藏长达28 d。用1 mM MeJA处理的桃果实在3 d货架期内延迟CI的发展，随后在贮藏后冷藏28 d（C28 dS 3）。MeJA处理后，桃果实中JA-Ile含量显著高于对照，这与缓解CI有关。RNA-Seq分析表明，与乙烯合成、果实软化、果肉布朗宁和香气相关的挥发性物质合成相关基因的表达发生了显著变化。与果实成熟相关的转录因子如PpNAC 1和JA信号转导如PpMYC2.2在MeJA处理的桃果实中表现出比对照更高的转录水平。在C28 dS 3冷藏后，有CI症状的桃果实的基因组甲基化水平高于MeJA处理的果实。转录丰度和启动子中DNA甲基化之间的反向模式观察到转录因子PpNAC 1和下游PpACS 1的乙烯合成，PpExp 1的软化，和PpAAT 1的挥发性酯和内酯的生产。总之，本研究不仅为MeJA通过转录组和DNA甲基化组的整合缓解果实冷害的调控机制提供了新的见解，也为桃果实冷藏后品质的保持奠定了基础。
Methyl jasmonate (MeJA) has been used to alleviate the chilling injury (CI) caused by postharvest cold storage of horticultural crops involving peach fruit. However, the molecular mechanism remains largely unknown. Here, we treated melting peach fruit (Prunus persica L. Batsch., cv. Hujingmilu) with different concentrations of MeJA, followed by storage at 0 degrees C up to 28 d. Peach fruit treated with 1 mM MeJA delayed the development of CI during 3 d shelf-life followed by 28 d cold after storage (C28dS3). The alleviated CI was associated with significant higher content of JA-Ile in peach fruit after MeJA treatment than controls. RNA-Seq revealed significant alternations in expression of genes related to ethylene synthesis, fruit softening, flesh browning and aroma-related volatile synthesis. Transcription factors associated with fruit ripening such as PpNAC1 and JA signaling such as PpMYC2.2 showed higher transcript levels in MeJA treated peach fruit than controls. Peach fruit with CI symptom had higher genome methylation level than MeJA treated fruit after cold storage at C28dS3. Reverse patterns between transcript abundance and DNA methylation in promoter were observed for transcription factor PpNAC1 and downstream PpACS1 for ethylene synthesis, PpExp1 for softening, and PpAAT1 for volatile ester and lactone production. Overall, in addition to providing new insights into the regulatory mechanism for MeJA in alleviating fruit chilling injury through integration of transcriptome and DNA methylome, our study also establishes a foundation for fruit quality maintaining after cold storage of peach fruit.