Subcellular localization and membrane association of SARS-CoV 3a protein.
Subcellular localization and membrane association of SARS-CoV 3a protein.
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DOI：
10.1016/j.virusres.2005.01.001
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发表时间：
2005-05
期刊：
影响因子：
5
通讯作者：
Cong Y
中科院分区：
文献类型：
--
作者：
Yuan X;Li J;Shan Y;Yang Z;Zhao Z;Chen B;Yao Z;Dong B;Wang S;Chen J;Cong Y
SARS-CoV 3a蛋白是SARS冠状病毒（SARS-CoV）的一种独特蛋白，在SARS-CoV感染细胞和SARS患者标本中均有发现。近年来的研究表明，3a蛋白可与SARS冠状病毒的M、E、S蛋白等多种结构蛋白特异性结合。在SARS-CoV感染的细胞中，3a蛋白定位于高尔基复合体。结果表明，3a蛋白主要定位于高尔基体，N端和C端有不同的标签。在不同的转染细胞中定位模式相似。对截短的3a蛋白的分析表明，3a可能含有三个跨膜区，第二或第三个跨膜区可能是高尔基体定位的区域。通过不同提取液的超离心实验，证实3a是一种完整的膜蛋白，并包埋在磷脂双分子层中。免疫荧光检测表明，共转染细胞中3a与M蛋白共定位于高尔基复合体。这些结果为进一步研究3a蛋白在SARS-CoV致病机制中的作用提供了新的思路。
SARS-CoV 3a protein was a unique protein of SARS coronavirus (SARS-CoV), which was identified in SARS-CoV infected cells and SARS patients’ specimen. Recent studies revealed that 3a could interact specifically with many SARS-CoV structural proteins, such as M, E and S protein. Expressed 3a protein was reported to localize to Golgi complex in SARS-CoV infected cells. In this study, it was shown that 3a protein was mainly located in Golgi apparatus with different tags at N- or C-terminus. The localization pattern was similar in different transfected cells. With the assay of truncated 3a protein, it was shown that 3a might contain three transmembrane regions, and the second or third region was properly responsible for Golgi localization. By ultra-centrifugation experiment with different extraction buffers, it was confirmed that 3a was an integral membrane protein and embedded in the phospholipid bilayer. Immunofluorescence assay indicated that 3a was co-localized with M protein in Golgi complex in co-transfected cells. These results provide a new insight for further study of the 3a protein on the pathogenesis of SARS-CoV.
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DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1074/jbc.m310875200
发表时间：
2004-01-16
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fan K;Wei P;Feng Q;Chen S;Huang C;Ma L;Lai B;Pei J;Liu Y;Chen J;Lai L
通讯作者：
Lai L
DOI：
10.1074/jbc.m009731200
发表时间：
2001-05-18
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lim KP;Liu DX
通讯作者：
Liu DX
影响因子：
5.4
作者：
Narayanan, K;Maeda, A;Makino, S
通讯作者：
Makino, S
影响因子：
5.4
作者：
de Haan, CAM;Vennema, H;Rottier, PJM
通讯作者：
Rottier, PJM