Analysis of the 16S-23S rRNA gene internal transcribed spacer region in Klebsiella species.
Analysis of the 16S-23S rRNA gene internal transcribed spacer region in Klebsiella species.
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克雷伯菌属 16S-23S rRNA 基因内转录间隔区分析。
DOI：
10.1128/jcm.00927-08
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发表时间：
2008
影响因子：
9.4
通讯作者：
Feng,Lu
中科院分区：
文献类型：
--
作者：
Wang,Min;Cao,Boyang;Yu,Qunfang;Liu,Lei;Gao,Qili;Wang,Lei;Feng,Lu
对肺炎克雷伯氏菌、臭克雷伯氏菌、肺炎克雷伯氏菌、鼻硬化克雷伯菌、催产克雷伯氏菌、植物克雷伯氏菌、陆地克雷伯氏菌和溶鸟克雷伯氏菌等6种克雷伯氏菌的16S-23S rRNA基因内转录间隔区(ITS区)进行了序列分析，并探讨了利用ITS区序列区分克雷伯氏菌种和亚种的可行性。对21株克雷伯氏菌代表菌株和11株克雷伯菌临床分离株的336个ITS序列进行了测序和分析。3种不同的ITS类型--ITSone(不含tRNA基因)、ITSglu[含tRNAGlu(Uuc)基因]和ITSile+Ala[含tRNAIle(GAU)和tRNAAla(UGC)基因]--在除Fork外的所有物种中被检测到。肺炎鼻硬化症，只有ITSlug和ITSile+Ala。肠杆菌科细菌中ITS的存在以前从未见过报道。每个ITS类型的长度和序列在物种内都高度保守，ITSone的同源性水平为0.961-1.000，ITSglu的同源性水平为0.967-1.000，ITSile+Ala的同源性水平为0.968-1.000。种间序列相似性ITSone为0.775~0.989，ITSglu为0.798~0.997，ITSile+Ala为0.712~0.985。发现了种间差异显著但种内多态度较低的区域；这些区域可能是设计种水平鉴定克雷伯氏菌的探针的靶标。基于ITS区的系统发育分析揭示了克雷伯氏菌种间的亲缘关系，与基于16S rRNA基因的亲缘关系相似。
The 16S-23S rRNA gene internal transcribed spacer (ITS) regions ofKlebsiellaspp., includingKlebsiella pneumoniaesubsp.pneumoniae, Klebsiella pneumoniaesubsp.ozaenae, Klebsiella pneumoniaesubsp.rhinoscleromatis, Klebsiella oxytoca, Klebsiella planticola, Klebsiella terrigena, andKlebsiella ornithinolytica, were characterized, and the feasibility of using ITS sequences to discriminateKlebsiellaspecies and subspecies was explored. A total of 336 ITS sequences from 21 representative strains and 11 clinical isolates ofKlebsiellawere sequenced and analyzed. Three distinct ITS types—ITSnone(without tRNA genes), ITSglu[with a tRNAGlu (UUC)gene], and ITSile+ala[with tRNAIle (GAU)and tRNAAla (UGC)genes]—were detected in all species except forK. pneumoniaesubsp.rhinoscleromatis, which has only ITSgluand ITSile+ala. The presence of ITSnoneinEnterobacteriaceaehad never been reported before. Both the length and the sequence of each ITS type are highly conserved within the species, with identity levels from 0.961 to 1.000 for ITSnone, from 0.967 to 1.000 for ITSglu, and from 0.968 to 1.000 for ITSile+ala. Interspecies sequence identities range from 0.775 to 0.989 for ITSnone, from 0.798 to 0.997 for ITSglu, and from 0.712 to 0.985 for ITSile+ala. Regions with significant interspecies variations but low intraspecies polymorphisms were identified; these may be targeted in the design of probes for the identification ofKlebsiellato the species level. Phylogenetic analysis based on ITS regions reveals the relationships amongKlebsiellaspecies similarly to that based on 16S rRNA genes.