YAP Expression in Normal and Neoplastic Breast Tissue: An Immunohistochemical Study
YAP Expression in Normal and Neoplastic Breast Tissue: An Immunohistochemical Study
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DOI：
10.1016/j.arcmed.2014.01.010
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发表时间：
2014-04-01
影响因子：
7.7
通讯作者：
Calderon-Garciduenas, Ana Laura
中科院分区：
文献类型：
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作者：
Jaramillo-Rodriguez, Yolanda;Cerda-Flores, Ricardo M.;Calderon-Garciduenas, Ana Laura
背景和目标。Yes相关蛋白（雅普）是一种转录因子，参与正常细胞的增殖、凋亡和癌变，但其在乳腺癌中的作用仍存在争议。我们进行了这项研究，比较雅普的表达免疫组织化学（IHC）在正常乳腺组织的妇女没有乳腺癌（BC）（对照），非肿瘤性乳腺组织的妇女癌症（内部控制）和在四个不同亚型的浸润性导管癌。通过IHC研究了17个对照和105个肿瘤病例（53个管腔A，15个管腔B，20个HER 2过表达和17个三阴性病例）。统计分析包括线性趋势的卡方检验（2）（扩展Mantel-Haenszel）。有40%的内部对照显示雅普在肌上皮细胞中表达，而在对照中表达为100%。在对照组中，3/17（17.6%）显示管腔细胞胞质染色。乳腺导管BC亚型之间的核表达存在显著差异。管腔A型有4%的阳性病例，
Background and Aims. Yes-associated protein (YAP) is a transcriptional factor involved in normal cell proliferation, apoptosis and carcinogenesis; however, its contribution to breast cancer (BC) is still controversial. We undertook this study to compare the expression of YAP by immunohistochemistry (IHC) in normal breast tissue of women without breast cancer (BC) (controls), non-neoplastic breast tissue in women with cancer (internal controls) and in four different subtypes of invasive ductal carcinoma.Methods. There were 17 controls and 105 tumor cases (53 luminal A, 15 luminal B, 20 overexpression of HER2 and 17 triple negative cases) studied by IHC. Statistical analysis included chi(2) for linear trend (Extended Mantel-Haenszel).Results. There were 40% of internal controls that showed expression of YAP in myoepithelial cells, whereas in controls expression was 100%. In controls, 3/17 (17.6%) showed cytoplasmic staining in luminal cells. There was a significant difference in nuclear expression between the ductal BC subtypes Luminal A had 4% of positive cases with