Photoreceptor decay over time and apoptosis in experimental retinal detachment
Photoreceptor decay over time and apoptosis in experimental retinal detachment
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DOI：
10.1007/bf01739640
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发表时间：
1997-05-01
影响因子：
2.7
通讯作者：
Seregard, S
中科院分区：
文献类型：
--
作者：
Berglin, L;Algvere, PV;Seregard, S
背景：关于视网膜脱离（RD）的外核层衰变的实际速率和方式的资料很少。我们使用实验兔模型来评估RD后细胞凋亡的存在和光感受器死亡率。该模型包括局部和稳定的视网膜气泡的产生，同时控制视网膜升高随时间的任何下降。方法：采用显微外科技术在新西兰大白兔神经视网膜下注射15%透明质酸钠0.05 ml制备RD。分别于第1、2、4、7、13和29天屠宰。对视网膜组织进行光镜和电子显微镜处理，并使用TUNEL技术的改进对片段DNA进行原位末端标记。在28只眼视网膜最大升高区进行感光细胞细胞核计数，另外1只眼进行缺口端标记。结果：DNA缺口端标记阳性、超微结构特征及坏死细胞缺失提示光感受器细胞凋亡。此外，随着时间的推移，光感受器细胞核会迅速地、几乎是线性地消失。在第14天，只有一半的原子核可以被辨认出来，而在29天之后，大约有十分之一的原子核还能被辨认出来。在3周的研究中，RD高度有统计学上显著但最小的下降。结论：家兔实验性RD。随着时间的推移，凋亡细胞死亡与光感受器细胞几乎线性消除有关。使用高粘性的透明质酸钠将神经视网膜与视网膜色素上皮分离，可使RD在4周内保持几乎恒定的高度。
Background: Data are scarce on the actual rate and mode of outer nuclear layer decay in retinal detachment (RD). We used an experimental rabbit model to assess the presence of apoptosis and rate of photoreceptor death following RD. This model included the creation of localized and stable retinal blebs, while controlling for any decline of retinal elevation over time. Methods: RD was produced in New Zealand white rabbits by injecting 0.05 ml of 15% sodium hyaluronate (Healon GV) under the neural retina using a microsurgical technique. Animals were killed at 1, 2, 4, 7, 13 and 29 days. Retinal tissue was processed for light and electron microscopy and for in situ end labeling of fragmented DNA using a modification of the TUNEL technique. Photoreceptor cell nuclei were counted in the RD areas of maximum retinal elevation of 28 eyes, and an additional I eyes were used for nick end labeling. Results: positive DNA nick end labeling, ultrastructural features and absence of necrotic cells indicated apoptotic photoreceptor cell death. Also, there was a rapid, almost linear elimination of photoreceptor nuclei over time. At 14 days only half of the number of nuclei were discernible, while approximately one tenth remained after 29 days. There was a statistically significant, but minimal decline in RD height over the 3 weeks of study. Conclusion: Following experimental RD in rabbits. apoptotic cell death is associated with an almost linear elimination of photoreceptor cells over time. The use of highly viscous sodium hyaluronate in separating the neural retina from the retinal pigment epithelium allows the RD to maintain a nearly constant height over a period of 4 weeks.