Requirement for the induced expression of a cell wall associated receptor kinase for survival during the pathogen response
Requirement for the induced expression of a cell wall associated receptor kinase for survival during the pathogen response
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DOI：
10.1046/j.1365-313x.1998.00092.x
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发表时间：
1998-04-01
期刊：
影响因子：
7.2
通讯作者：
Kohorn, BD
中科院分区：
文献类型：
--
作者：
He, ZH;He, DZ;Kohorn, BD
被子植物的病原体感染必须依赖于细胞外基质（ECM）与入侵因子之间的相互作用，并可能伴随着细胞外基质与细胞质之间的信号传递。拟南芥细胞壁相关受体激酶（Wak1）具有与ECM紧密相关的氨基末端结构域，跨越质膜并具有细胞质蛋白激酶结构域。Wak1的表达是在拟南芥植物被病原体感染时，或者当外源水杨酸盐（SA）或其类似物2,2-二氯异烟酸（INA）刺激病原体反应时诱导的。Wak1的诱导需要正调节因子NPR1/NIM1。因此Wak1是一种病原体相关（PR）蛋白。Wak1的一个反义等位基因和一个显性负等位基因的表达表明，在受INA刺激时，植物需要诱导Wak1的表达才能存活。整个Wak1的异位表达，或单独的激酶结构域，可以提供对致命SA水平的抗性。这些实验表明Wak1和其他PR蛋白的表达可以保护植物免受病原菌应答过程中的有害影响。这些结果提供了一种蛋白激酶之间的直接联系，这种蛋白激酶可以介导来自ECM的信号，与病原体感染引发的事件有关。
Pathogen infection of angiosperms must rely on some interaction between the extracellular matrix (ECM) and the invading agent, and may be accompanied by signaling between the ECM and cytoplasm. An Arabidopsis cell wall associated receptor kinase (Wak1) has an amino-terminal domain that is tightly associated with the ECM, spans the plasma membrane and has a cytoplasmic protein kinase domain. Wak1 expression is induced when Arabidopsis plants are infected with pathogen, or when the pathogen response is stimulated either by exogenous salicylate (SA) or its analog 2,2-dichloroisonicotinic acid (INA). This Wak1 induction requires the positive regulator NPR1/NIM1. Thus Wak1 is a pathogen-related (PR) protein. Expression of an antisense and a dominant negative allele of Wak1 shows that induced expression of Wak1 is needed for a plant to survive if stimulated by INA. Ectopic expression of the entire Wak1, or the kinase domain alone, can provide resistance to otherwise lethal SA levels. These experiments suggest that Wak1 expression and other PR proteins are protecting plants from detrimental effects incurred during the pathogen response. These results provide a direct link between a protein kinase that could mediate signals from the ECM, to the events that are precipitated by a pathogen infection.