The Nuclear Export Signal of Splicing Factor Uap56p Interacts with Nuclear Pore-associated Protein Rae1p for mRNA Export in Schizosaccharomyces pombe*
The Nuclear Export Signal of Splicing Factor Uap56p Interacts with Nuclear Pore-associated Protein Rae1p for mRNA Export in Schizosaccharomyces pombe*
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DOI：
10.1074/jbc.m609727200
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发表时间：
2007-06
影响因子：
4.8
通讯作者：
A. Thakurta;Saravana P Selvanathan;Andrew D. Patterson;G. Gopal;R. Dhar
中科院分区：
文献类型：
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作者：
A. Thakurta;Saravana P Selvanathan;Andrew D. Patterson;G. Gopal;R. Dhar
哺乳动物UAP 56或其在酿酒酵母中的同源物Sub 2 p是ATP依赖性RNA解旋酶家族的成员，并且是mRNA的剪接和核输出所必需的。以前我们表明，在裂殖酵母粟酒裂殖酵母Uap 56 p是关键的mRNA输出。它连接mRNA衔接子Mlo 3 p，一种在S.酿酒酵母或Aly在哺乳动物中，核孔相关的mRNA输出因子Rae 1 p。在这项研究中，我们表明，在相反的S。酿酒酵母Uap 56 p;前体mRNA剪接不需要粟酒裂殖酵母。Uap 56 p的RNA解旋酶功能不是mRNA输出所必需的。然而，Uap 56 p的RNA结合基序对于mRNA的核输出至关重要。在Uap 56 p中，我们确定了核输入和输出信号，这些信号可能允许它在细胞核和细胞质之间穿梭。我们发现Uap 56 p通过其核输出信号直接与Rae 1 p相互作用，这种相互作用对于Uap 56 p的核输出活性以及输出mRNA至关重要。RNA结合和在细胞核和细胞质之间穿梭的能力是mRNA输出载体如HIV-Rev的重要特征。粟球。
Mammalian UAP56 or its homolog Sub2p in Saccharomyces cerevisiae are members of the ATP-dependent RNA helicase family and are required for splicing and nuclear export of mRNA. Previously we showed that in Schizosaccharomyces pombe Uap56p is critical for mRNA export. It links the mRNA adapter Mlo3p, a homolog of Yra1p in S. cerevisiae or Aly in mammals, to nuclear pore-associated mRNA export factor Rae1p. In this study we show that, in contrast to S. cerevisiae, Uap56p in S. pombe is not required for pre-mRNA splicing. The putative RNA helicase function of Uap56p is not required for mRNA export. However, the RNA-binding motif of Uap56p is critical for nuclear export of mRNA. Within Uap56p we identified nuclear import and export signals that may allow it to shuttle between the nucleus and the cytoplasm. We found that Uap56p interacts with Rae1p directly via its nuclear export signal, and this interaction is critical for the nuclear export activity of Uap56p as well as for exporting mRNA. RNA binding and the ability to shuttle between the nucleus and cytoplasm are important features of mRNA export carriers such as HIV-Rev. Our results suggest that Uap56p could function similarly as an export carrier of mRNA in S. pombe.