Plasma cysteine/cystine redox couple disruption in animal models of temporal lobe epilepsy.
Plasma cysteine/cystine redox couple disruption in animal models of temporal lobe epilepsy.
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DOI：
10.1016/j.redox.2016.05.004
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发表时间：
2016-10
期刊：
影响因子：
11.4
通讯作者：
Patel M
中科院分区：
文献类型：
--
作者：
Liang LP;Patel M
目前，癫痫领域缺乏基于外周血的生物标志物，可以预测癫痫损伤后慢性癫痫发作或进展。硫醇/二硫化物比率已显示为评估组织和血浆中的全身氧化还原电位提供了灵敏的方法。在这项研究中，我们利用了一种快速，简单，可靠的方法，同时测定几个巯基氨基酸在血浆中使用高效液相色谱电化学检测在红藻氨酸（KA）和毛果芸香碱癫痫大鼠模型。与GSH和GSSG水平相比，KA和匹鲁卡品注射大鼠血浆中半胱氨酸（Cys）水平在48 h后分别下降42%和62%，胱氨酸（Cys）水平分别上升46%和23%。在慢性癫痫大鼠中，KA和匹罗卡品分别使血浆半胱氨酸降低40.4%和37.7%，血浆GSSG升高33.8%和35.0%。KA或毛果芸香碱后60分钟用催化抗氧化剂治疗大鼠，在两种模型中48 h时间点显著减弱血浆Cys/Cyss比率的降低。这些观察结果表明，血浆中半胱氨酸和Cys/Cys比值的降低可能作为颞叶癫痫的氧化还原生物标志物。高效液相色谱法测定大鼠血浆中含巯基氨基酸。化学惊厥药诱发癫痫急性期和慢性期血浆半胱氨酸/胱氨酸水平降低血浆半胱氨酸/胱氨酸的消耗可以通过催化抗氧化剂来改善。血浆半胱氨酸/胱氨酸的测量可以作为癫痫的氧化还原生物标志物。
Currently the field of epilepsy lacks peripheral blood-based biomarkers that could predict the onset or progression of chronic seizures following an epileptogenic injury. Thiol/disulfide ratios have been shown to provide a sensitive means of assessing the systemic redox potential in tissue and plasma. In this study, we utilized a rapid, simple and reliable method for simultaneous determination of several thiol-containing amino acids in plasma using HPLC with electrochemical detection in kainic acid (KA) and pilocarpine rat models of epilepsy. In contrast to GSH and GSSG levels, the levels of cysteine (Cys) were decreased by 42% and 62% and cystine (Cyss) were increased by 46% and 23% in the plasma of KA- and pilocarpine-injected rats, respectively after 48 h. In chronically epileptic rats, plasma cysteine was decreased by 40.4% and 37.7%, and plasma GSSG increased by 33.8% and 35.0% following KA and pilocarpine, respectively. Treatment of rats with a catalytic antioxidant, 60 min after KA or pilocarpine significant attenuated the decrease of plasma Cys/Cyss ratios at the 48 h time point in both models. These observations suggest that the decreased cysteine and ratio of Cys/Cyss in plasma could potentially serve as redox biomarkers in temporal lobe epilepsy. HPLC method for determination thiol-containing amino acids in rat plasma. Decreased plasma cysteine/cystine in acute and chronic stages of chemoconvulsant-induced epilepsy. Depletion of plasma cysteine/cystine can be ameliorated by a catalytic antioxidant. Measurement of plasma cysteine/cystine could serve as redox biomarkers in epilepsy.