Yeast RNA polymerase II transcription reconstituted with purified proteins

Yeast RNA polymerase II transcription reconstituted with purified proteins
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DOI：
10.1006/meth.1997.0473
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发表时间：
1997-07-01
期刊：
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
影响因子：
--
通讯作者：
Kornberg, RD
Kornberg, RD
中科院分区：
其他
文献类型：
--
作者：
Myers, LC;Leuther, K;Kornberg, RD

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介绍了一种完全定义的酵母RNA聚合酶II转录系统的制备方案，该转录系统基本上由纯的TFIIB、-E、-F和-H、TATA结合蛋白、RNA聚合酶II和转录调节的介体组成。该系统由44个多肽组成，能够在迄今测试的十几个酵母和哺乳动物启动子中的任何一个上启动转录，并对各种转录激活蛋白做出反应。(C)1997年学术出版社。
Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins. (C) 1997 Academic Press.