The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides
The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides
复制标题
DOI：
10.1016/j.biomaterials.2011.02.013
复制
发表时间：
2011-06-01
期刊：
影响因子：
14
通讯作者：
Kizhakkedathu, Jayachandran N.
中科院分区：
文献类型：
--
作者：
Gao, Guangzheng;Lange, Dirk;Kizhakkedathu, Jayachandran N.
植入物表面的细菌定植和随后的感染是许多留置器械失效的最常见原因之一。已经尝试了几种方法，包括植入物上的抗菌和抗菌洗脱涂层;然而，这些方法都没有在体内成功。在这里，我们报告了一种基于聚合物刷的植入物涂层，该涂层无毒、抗微生物和抗生物膜。这些涂层由共价接枝的亲水性聚合物链与一系列优化的抗菌肽（AMP）共轭组成。这些拴系的AMP在体外和体内保持了优异的广谱抗微生物活性。我们发现这种特殊结构的坚固涂层在抵抗生物膜形成方面非常有效，并且生物膜抗性取决于缀合肽的性质。该涂层对成骨样细胞没有毒性，在人血液中显示出不显著的血小板活化和粘附以及补体活化。由于此类涂层可以应用于大多数当前使用的植入物表面，因此我们的方法对于开发抗感染植入物具有巨大潜力。(C)2011爱思唯尔有限公司保留所有权利。
Bacterial colonization on implant surfaces and subsequent infections are one of the most common reasons for the failure of many indwelling devices. Several approaches including antimicrobial and antibioticeluting coatings on implants have been attempted; however, none of these approaches succeed in vivo. Here we report a polymer brush based implant coating that is non-toxic, antimicrobial and biofilm resistant. These coating consists of covalently grafted hydrophilic polymer chains conjugated with an optimized series of antimicrobial peptides (AMPs). These tethered AMPs maintained excellent broad spectrum antimicrobial activity in vitro and in vivo. We found that this specially structured robust coating was extremely effective in resisting biofilm formation, and that the biofilm resistance depended on the nature of conjugated peptides. The coating had no toxicity to osteoblast-like cells and showed insignificant platelet activation and adhesion, and complement activation in human blood. Since such coatings can be applied to most currently used implant surfaces, our approach has significant potential for the development of infection-resistant implants. (C) 2011 Elsevier Ltd. All rights reserved.