Structural biology of the fibres of the collagenous and elastic systems
Structural biology of the fibres of the collagenous and elastic systems
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DOI：
10.1006/cbir.1996.0004
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发表时间：
1996-01-01
影响因子：
3.9
通讯作者：
Montes, GS
中科院分区：
文献类型：
--
作者：
Montes, GS
通过将每种纤维类型的典型超微结构图像与使用光学显微镜选择性染色技术的研究进行比较，可以在组织切片中具体证明胶原和弹性系统的不同类型的纤维。提出了一种实用的操作方法，包括推荐的染色程序和结果的解释。提供显微照片和表格来总结差异程序。当通过银浸渍技术研究时，网状纤维表现出明显的嗜银性，并且当借助天狼星偏振法检查时，显示为弱双折射、绿色纤维的薄网状结构。此外，电子显微镜研究表明，网状纤维由少量细胶原原纤维组成，与大量较粗的原纤维形成对比，后者可以在超微结构上定位于通过组织化学方法表征的非嗜银粗胶原纤维的部位。弹性系统的三种不同纤维类型属于连续系列：oxytalan-elaunin-elastic（所有纤维类型都包含微原纤维的集合，并且按给定的顺序，弹性蛋白的量逐渐增加）。三种不同类型的弹性系统纤维具有不同的染色特征和超微结构模式。从超微结构上看，特征弹性纤维由两种形态不同的成分组成：位于中心的无定形均质弹性蛋白实心圆柱体，周围环绕着管状微原纤维。 oxytalan纤维由一束微原纤维组成，与弹性纤维微原纤维相同；无非晶材料。在弹性纤维中，分散的无定形材料（弹性蛋白）混合在微纤维之间。 (C) 1996 学术出版社有限公司
The different types of fibres of the collagenous and elastic systems can be demonstrated specifically in tissue sections by comparing the typical ultrastructural picture of each of the fibre types with studies using selective staining techniques for light microscopy. A practical modus operandi, which includes the recommended staining procedures and interpretation of the results, is presented. Micrographs and tables are provided to summarize the differential procedures. Reticulin fibres display a distinct argyrophilia when studied by means of silver impregnation techniques, and show up as a thin meshwork of weakly birefringent, greenish fibres when examined with the aid of the Picrosirius-polarization method. In addition, electron-microscopic studies showed that reticulin fibres are composed of a small number of thin collagen fibrils, contrasting with the very many thicker fibrils that could be localized ultrastructurally to the sites where non-argyrophilic, coarse collagen fibres had been characterized by the histochemical methods used. The three different fibre types of the elastic system belong to a continuous series: oxytalan-elaunin-elastic (all of the fibre types comprising collections of microfibrils with, in the given sequence, increasing amounts of elastin). The three distinct types of elastic system fibres have different staining characteristics and ultrastructural patterns. Ultrastructurally, a characteristic elastic fibre consists of two morphologically different components: a centrally located solid cylinder of amorphous and homogeneous elastin surrounded by tubular microfibrils. An oxytalan fibre is composed of a bundle of microfibrils, identical to the elastic fibre microfibrils; without amorphous material. In elaunin fibres, dispersed amorphous material (elastin) is intermingled among the microfibrils. (C) 1996 Academic Press Limited