Methyl salicylate delays peel yellowing of 'Zaosu' pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure
Methyl salicylate delays peel yellowing of 'Zaosu' pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure
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水杨酸甲酯通过调节叶绿素代谢和维持叶绿体超微结构延缓‘早酥梨’贮藏期间果皮黄变
DOI：
10.1002/jsfa.9737
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发表时间：
2019
影响因子：
4.1
通讯作者：
Wang Junjie
中科院分区：
文献类型：
--
作者：
Zhang Huaiyu;Wang Rui;Wang Tian;Fang Chunxia;Wang Junjie
在某些栽培品种中，叶绿素分解导致的黄化对食物供应和健康有直接和负面的影响。早酥梨（Pyrus bretschneideriodd.）一种在中国的商业亚洲梨品种，在收获后在室温下储存时迅速变黄。为探索延缓或抑制叶绿素降解的技术，以早酥梨为试材，研究了水杨酸甲酯（梅萨）对早酥梨果实贮藏期间黄化的影响。结果表明：与对照相比，0.05 mmol L− 1 MeSA处理延缓了早酥梨果实总叶绿素、叶绿素a和叶绿素B含量的下降，并保持了较完整的叶绿体和较小的质体数量。水杨酸甲酯抑制叶绿素酶、叶绿素降解过氧化物酶、镁脱螯合酶和脱镁叶绿素酶的活性，抑制NYC、NOL、CLH、SGR、PPH、PAO和RCCR的表达。结果还表明，叶绿素a向脱镁叶绿酸a的转化可以通过两条途径进行，并且‘早酥梨’中存在叶绿素a分解的替代途径。© 2019化学工业协会
BACKGROUNDIn some cultivars, yellowing resulting from chlorophyll breakdown has a direct and negative effect on food supply and health. The ‘Zaosu’ pear (Pyrus bretschneideriRehd.), a commercial Asian pear cultivar in China, rapidly turns yellow when stored at room temperature after harvest. To develop techniques that delay or suppress chlorophyll degradation, the effects of methyl salicylate (MeSA) on yellowing in ‘Zaosu’ pear fruit during storage were evaluated.RESULTSCompared with the untreated fruit, the application of 0.05 mmol L−1MeSA delayed the decline of the total chlorophyll, chlorophyll a and chlorophyll b content, and maintained more intact chloroplasts with fewer and smaller plastoglobuli. Methyl salicylate suppressed enzyme activities, including chlorophyllase, chlorophyll‐degrading peroxidase, Mg dechelatase, and pheophytinase, and the expression levels ofNYC,NOL,CLH,SGR,PPH,PAOandRCCRin treated fruit.CONCLUSIONMethyl salicylate could delay chlorophyll breakdown in the fruit. The results also suggested that the conversion from chlorophyll a to pheophorbide a could proceed via two pathways, and that alternative pathways for the breakdown of chlorophyll a exist in ‘Zaosu’ pears. © 2019 Society of Chemical Industry