Effect of rapid shallow breathing on the distribution of 18O-labeled ozone reaction product in the respiratory tract of the rat.
Effect of rapid shallow breathing on the distribution of 18O-labeled ozone reaction product in the respiratory tract of the rat.
复制标题
快速浅呼吸对大鼠呼吸道内18O标记臭氧反应产物分布的影响。
DOI：
10.1080/08958370490264852
复制
发表时间：
2004
影响因子：
2.1
通讯作者：
Schelegle,EdwardS
中科院分区：
文献类型：
--
作者：
Alfaro,MarioF;Putney,Lei;Tarkington,BrianK;Hatch,GaryE;Hyde,DallasM;Schelegle,EdwardS
我们研究了呼吸模式对呼吸道内臭氧反应产物含量的影响。34只麻醉的雄性Wistar大鼠暴露于1.0 ppm的氧-18（18 O）-标记臭氧中2 h，使用双室负压通气系统。频率设定为80（n = 9）、120（n = 7）、160（n = 8）或200（n = 10）次呼吸/分钟（bpm），而潮气量（Vt）设定为提供72.8 ml/min/100 g体重的恒定分钟通气量。气道采样从中层气管和主支气管和脑实质的头部和尾部的右叶。18 O含量在每个气道样本进行了定量和归一化的表面积。在频率上，有显着更大（p <。05）气管和支气管（传导气道上皮）中的18 O含量与实质取样部位相比。气管18 O含量在80和160 bpm之间降低，但随后在200 bpm时增加。相比之下，在右侧头侧和尾侧支气管部位，18 O含量在80和200 bpm之间逐渐增加。与80、120和160 bpm相比，200 bpm时右颅实质18 O含量降低。右尾实质18 O含量相对恒定的所有呼吸频率。我们的结论是，从80到160 bpm的快速浅呼吸的发展导致减少沉积的O3在气管中，而只有轻微的影响，以臭氧沉积在实质中提供的短和长的气道路径。
We examined the effect of breathing pattern on ozone reaction product content within the respiratory tract. Thirty-four anesthetized, male Wistar rats were exposed to oxygen-18 (18O)-labeled ozone at 1.0 ppm for 2 h using a dual-chamber, negative-pressure ventilation system. Frequency was set at 80 (n = 9), 120 (n = 7), 160 (n = 8), or 200 (n = 10) breaths per minute (bpm), while tidal volume (Vt) was set to provide a constant minute ventilation of 72.8 ml/min/100 g body weight. Airways sampled were from the midlevel trachea and the mainstem bronchi and parenchyma of the cranial and caudal right lobes.18O content in each airway sample was quantified and normalized to surface area. Across frequencies, there was significantly greater (p <. 05)18O content in the trachea and bronchi (conducting airway epithelium) compared to the parenchyma sampling sites. Tracheal18O content decreased between 80 and 160 bpm, but then underwent an increase at 200 bpm. In comparison,18O content gradually increased between 80 and 200 bpm at the right cranial and caudal bronchi sites. Right cranial parenchymal18O content decreased at 200 bpm compared to 80, 120, and 160 bpm. Right caudal parenchymal18O content was relatively constant over all breathing frequencies. We concluded that the development of rapid shallow breathing from 80 to 160 bpm results in a reduced deposition of O3in the trachea, while only mildly affecting to ozone deposition in parenchyma supplied by short and long airway paths.