Developmentally regulated stabilization of neuronal intermediate filaments in rat cerebral cortex
Developmentally regulated stabilization of neuronal intermediate filaments in rat cerebral cortex
复制标题
大鼠大脑皮层神经元中间丝的发育调节稳定
DOI：
10.1016/s0304-3940(97)00425-4
复制
发表时间：
1997
影响因子：
2.5
通讯作者：
W. Mushynski
中科院分区：
文献类型：
--
作者：
B. Giasson;W. Mushynski
神经元中间丝蛋白的表达和Triton X-100（Triton）的溶解度测定在发育中的大鼠大脑皮层。α-internexin的表达水平从胚胎第15天（E15）到出生后第15天（P15）没有变化，而中等大小的神经丝亚基的表达在此期间持续增加，伴随着这两种蛋白质的Triton溶解度的降低。低分子量神经丝亚基，首先在P2几乎检测不到，从其溶解度可以测定的初始时间点（P5）到P15，在Triton中基本不溶。在E15大脑皮层培养的神经元中，获得了类似的表达模式和Triton溶解度曲线的神经元中间丝蛋白。这些结果表明，α-internexin比神经丝蛋白更早表达，从而在早期发育的大脑中提供更具可塑性的网络。神经丝蛋白的掺入显然导致在成熟神经元中发现的更稳定的中间丝网络的形成。
The expression and Triton X-100 (Triton) solubility of neuronal intermediate filament proteins were determined in the developing rat cerebral cortex. The level of expression of α-internexin was unchanged from embryonic day 15 (E15) to postnatal day 15 (P15), whereas expression of the mid-sized neurofilament subunit increased continually during this interval concomitant with a reduction in Triton solubility of the two proteins. The low molecular weight neurofilament subunit, first barely detected at P2, was largely insoluble in Triton from the initial time point that its solubility could be assayed, at P5, to P15. Similar expression patterns and Triton solubility profiles were obtained for neuronal intermediate filament proteins in cultured neurons from E15 cerebral cortex. These results suggest that α-internexin is expressed earlier than neurofilament proteins to provide a more plastic network in the early developing brain. The incorporation of neurofilament proteins apparently results in the formation of the more stable intermediate filament network found in mature neurons.