T-box family reunion.
T-box family reunion.
复制标题
T-box家庭团聚。
DOI：
10.1016/s0168-9525(97)01144-x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Papaioannou,VE
中科院分区：
文献类型：
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作者：
Papaioannou,VE
窃窃私语变成了咆哮。最近一系列关于T-box家族基因功能的论文，在像外星人和人类这样不同的生物体中，已经开始兑现这个新型转录因子家族在胚胎发育中发挥核心作用的承诺。起初，在60多年的时间里，只有小鼠基因Bracbyu或T，这是一个具有深远发育影响的基因，如果进化起源模糊的话。继1990年克隆该基因（参考文献1）之后，Brachyuo.，正向原木开始在其他物种中出现。然后，在1992年，当Pflugfelder和他的同事检测到Brachyu和果蝇基因omb之间共享的同源结构域时，第一次提出了家族关系，这显然不是Brachyu O '直系同源物。在接下来的几年中，在小鼠、秀丽隐杆线虫和人类的基因组中发现了分散的近亲和更远的亲属3 -7，并且随着关于T-box基因的最新报道的证明，发现的趋势继续。T-box基因家族的定义特征是保守序列的区域，称为T-box，其主要对应于编码蛋白质16的DNA结合结构域的区域。可以理解的是，只要基因产物继续作为DNA结合蛋白发挥作用，该区域就会高度保守。因此，该区域氨基酸序列的相似性提供了遗传证据，可用于估计进化距离，从而确定基因复制事件何时发生与物种分化有关。比如说
The whispers have become a roar. A recent flurry of papers that bear on the functions of genes of the T-box family, in organisms as diverse as Xen-opusand humans, has begun to fulfill the promise that this novel family of transcription factors plays central roles in embryonic development. At first, and for over 60 years, there was only the mouse gene Bracbyu~ or T, a gene with profound developmental effects, if obscure evolutionary origins. Following the cloning of this gene in 1990 (Ref. 1), Brachyuo., ortho-logs began to tum up in other species. Then, in 1992, came the first suggestion of family ties when Pflugfelder and colleagues z detected a homology domain shared between Brachyu~, and a Drosophila gene, omb, that was clearly not a Brachyu O'ortholog. During the next few years, near cousins and more-distant relatives were found scattered around the genomes of the mouse, Caenorhabditis elega ns and humans3-7, and the trend of discovery continues as the latest crop of reports on T-box genes attestsS-15.The defining feature of the T-box gene family is a region of conserved sequence, called the T-box, which corresponds largely to the region encoding the DNA-binding domain of the protein 16. It is understandable that this region would have been highly conserved as long as the gene products continued to function as DNA-binding proteins. Consequently, the similarity" of amino acid sequence in this region provides genetic evidence that can be used to estimate evolutionary distance and, thus, to determine when gene duplication events took place in relation to the divergence of species. For example, the