MiR-140-5p promotes osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post-fracture healing of mice.
MiR-140-5p promotes osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post-fracture healing of mice.
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DOI：
10.1002/cbf.3585
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发表时间：
2020-12
影响因子：
3.6
通讯作者：
Liu J
中科院分区：
文献类型：
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作者：
Jiao J;Feng G;Wu M;Wang Y;Li R;Liu J
MiR - 140 - 5p在正常骨折愈合中高表达，但其在组织-骨愈合中的具体作用和机制很少报道。因此，本研究探讨了miR - 140 - 5p对组织-骨愈合的影响。采用克隆形成实验、流式细胞术、茜素红S染色和油红O染色研究小鼠胚胎骨髓间充质干细胞C3H10T1/2的生物学特性。将MiR‐140‐5p模拟物转染到成骨培养基（OS）处理过的C3H10T1/2细胞中，研究MiR‐140‐5p对成骨分化的影响。建立MiR‐140‐5p转基因小鼠模型和转基因骨折模型，采用苏木精、伊红染色和计算机断层扫描检测MiR‐140‐5p对骨组织成骨分化、骨密度（BMD）和骨量的影响。定量实时聚合酶链反应测定骨钙素、分化相关基因（Runx2、ALP、Spp1和Bglap3）和miR‐140‐5p的表达。C3H10T1/2细胞显示出形成成骨细胞、脂肪细胞的克隆分化能力，其表型包括CD44、CD90.1和Sca‐1，但不包括CD45造血干细胞标记物。miR - 140 - 5p的过表达促进了OS处理的C3H10T1/2细胞分化相关基因的表达和钙沉积。MiR‐140‐5p增加了MiR‐140‐5p转基因骨折小鼠骨钙素、骨密度和骨量的表达，促进了骨愈合。MiR‐140‐5p促进小鼠胚胎骨髓间充质干细胞的成骨分化和小鼠骨折后愈合。C3H10T1/2细胞显示出形成成骨细胞、脂肪细胞及其表型（包括CD44、CD90.1和Sca‐1）的克隆分化能力，但不包括CD45造血干细胞标记物。miR - 140 - 5p的过表达促进了成骨培养基处理的C3H10T1/2细胞分化相关基因的表达和钙沉积。MiR‐140‐5p增加了MiR‐140‐5p转基因骨折小鼠骨钙素的表达、骨矿物质密度和骨量，促进了骨愈合。我们的研究结果表明miR - 140 - 5p促进小鼠胚胎骨髓间充质干细胞的成骨分化和小鼠骨折后的愈合，这可能是治疗骨折和促进骨愈合的治疗靶点。
MiR‐140‐5p is high expressed in normal fracture healing, but its specific role and mechanism in tissue‐to‐bone healing are rarely reported. Therefore, this study investigated the effects of miR‐140‐5p on tissue‐to‐bone healing. Clone formation experiment, flow cytometry, Alizarin Red S Staining and Oil Red O Staining were performed to investigate the biological characteristics of mouse embryonic bone marrow mesenchymal stem cells C3H10T1/2. MiR‐140‐5p mimic was transfected into osteogenic medium (OS)‐treated C3H10T1/2 cells to investigate the effects of miR‐140‐5p on osteogenic differentiation. MiR‐140‐5p transgenic mouse model and the transgenic fracture model were established, and the effects of miR‐140‐5p on osteogenic differentiation, bone mineral density (BMD) and bone mass of bone tissues were detected by haematoxylin and eosin staining and computed tomography scan. The expressions of osteocalcin, differentiation‐related genes (Runx2, ALP, Spp1 and Bglap3) and miR‐140‐5p were determined by quantitative real‐time polymerase chain reaction. C3H10T1/2 cells showed the abilities of forming cloned differentiation of osteogenesis, fat cells, and its phenotypes including CD44, CD90.1 and Sca‐1 but excluding CD45 haematopoietic stem cell marker. Overexpression of miR‐140‐5p promoted the expressions of differentiation‐related genes and calcium deposition of OS‐treated C3H10T1/2 cells. MiR‐140‐5p increased the expression of osteocalcin, BMD and bone mass and promoted bone healing of miR‐140‐5p‐transgenic mice with fracture. MiR‐140‐5p promoted osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post‐fracture healing in mice. C3H10T1/2 cells showed the abilities of forming cloned differentiation of osteogenesis, fat cells and its phenotypes including CD44, CD90.1 and Sca‐1 but excluding CD45 haematopoietic stem cell marker. Overexpression of miR‐140‐5p promoted the expressions of differentiation‐related genes and calcium deposition of osteogenic medium‐treated C3H10T1/2 cells. MiR‐140‐5p increased the expression of osteocalcin and bone mineral density and bone mass and promoted bone healing of miR‐140‐5p‐transgenic mice with fracture. Our results showed that miR‐140‐5p promoted osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post‐fracture healing in mice, which may be a therapeutic target for treating fractures and promoting bone healing.
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影响因子：
3.7
作者：
Eguchi T;Watanabe K;Hara ES;Ono M;Kuboki T;Calderwood SK
通讯作者：
Calderwood SK
Runx2/miR-3960/miR-2861正反馈环负责血管平滑肌细胞的成骨转分化
影响因子：
--
作者：
Xia ZY;Hu Y;Xie PL;Tang SY;Luo XH;Liao EY;Chen F;Xie H
通讯作者：
Xie H
DOI：
10.1016/j.bbamcr.2014.06.004
发表时间：
2014-09-01
影响因子：
5.1
作者：
Qiu, Weimin;Kassem, Moustapha
通讯作者：
Kassem, Moustapha
影响因子：
4.2
作者：
Mahboudi, Hossein;Soleimani, Masoud;Kazemi, Bahram
通讯作者：
Kazemi, Bahram
DOI：
10.1261/rna.049148.114
发表时间：
2015-08
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Laxman N;Rubin CJ;Mallmin H;Nilsson O;Pastinen T;Grundberg E;Kindmark A
通讯作者：
Kindmark A