THE HUMAN TCF-1 GENE ENCODES A NUCLEAR DNA-BINDING PROTEIN UNIQUELY EXPRESSED IN NORMAL AND NEOPLASTIC T-LINEAGE LYMPHOCYTES
THE HUMAN TCF-1 GENE ENCODES A NUCLEAR DNA-BINDING PROTEIN UNIQUELY EXPRESSED IN NORMAL AND NEOPLASTIC T-LINEAGE LYMPHOCYTES
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DOI：
10.1182/blood.v86.8.3050.bloodjournal8683050
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发表时间：
1995-10-15
期刊：
影响因子：
20.3
通讯作者：
CLEVERS, H
中科院分区：
文献类型：
--
作者：
CASTROP, J;VANWICHEN, D;CLEVERS, H
The TCF-1 gene encodes a putative transcription factor with affinity for a sequence motif occurring in a number of T-cell enhancers. TCF-1 mRNA was originally found to be expressed in a T cell-specific fashion within a set of human and mouse cell lines. In contrast, expression reportedly occurs in multiple nonlymphoid tissues during murine embryogenesis. We have now raised a monoclonal antibody to document expression and biochemistry of the human TCF-1 protein. As expected, the TCF-1 protein was detectable only in cell lines of T lineage. Its expression was always restricted to the nucleus. Immunohistochemistry on a panel of human tissues revealed that the TCF-1 protein was found exclusively in thymocytes and in CD3(+) T cells in peripheral lymphoid tissues. Western blotting yielded a set of bands ranging from 25 kD to 55 kD, resulting from extensive alternative splicing. The TCF-1 protein was detectable in all samples of a set of 22 T-cell malignancies of various stages of maturation, but was absent from a large number of other hematologic neoplasms. These observations imply a T cell-specific function for TCF-1, a notion corroborated by recent observations on Tcf-1 knock-out mice. In addition, these results indicate that nuclear TCF-1 expression can serve as a pan-T-lineage marker in the diagnosis of lymphoid malignancies. (C) 1995 by The American Society of Hematology.
