Identification of plasmids by PCR-based replicon typing
Identification of plasmids by PCR-based replicon typing
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DOI：
10.1016/j.mimet.2005.03.018
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发表时间：
2005-12-01
影响因子：
2.2
通讯作者：
Threlfall, EJ
中科院分区：
文献类型：
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作者：
Carattoli, A;Bertini, A;Threlfall, EJ
流行病学的重要性，追踪质粒赋予耐药性，促使我们开发了一种PCR方法的基础上复制子（inc/repPCR）的主要质粒不相容组之间的肠杆菌。设计18对引物进行5次多重PCR和3次单重PCR，识别FIA、FI B、FIC、HI 1、H12、I1-1 γ、L/M、N、P、W、T、A/C、K、B/O、X、Y、F和FIIA。在收集的61个参考质粒和在意大利分离的20个不同血清型的肠道沙门氏菌菌株上检测了该方法的特异性。结果表明，inc/repPCR方法在检测参考质粒上的复制子方面表现出较高的特异性和灵敏度，并且还揭示了不同血清型流行病学无关的沙门氏菌分离株中存在重复性和常见质粒。这些结果表明，该方法是潜在的适用于大量的菌株，以跟踪特定的多药耐药质粒在不同环境中的扩散。(c)2005 Elsevier B. V.保留所有权利。
The epidemiological importance of tracing plasmids conferring drug resistance prompted us to develop a PCR method based on replicons (inc/rep PCR) of the major plasmid incompatibility groups among Enterobacteriaceae. Eighteen pairs of primers were designed to perform 5 multiplex- and 3 simplex-PCRs, recognizing FIA, FIB, FIC, HI 1, H12, I1-1 gamma, L/M, N, P, W, T, A/C, K, B/O, X, Y, F, and FIIA. The specificity of the method was tested on a collection of 61 reference plasmids and on 20 Salmonella enterica strains of different serotypes isolated in Italy. Results indicated that the inc/rep PCR method demonstrates high specificity and sensitivity in detecting replicons on reference plasmids and also revealed the presence of recurrent and common plasmids in epidemiologically unrelated Salmonella isolates of different serotypes. These results suggest that the method is potentially applicable to a large number of strains to trace the diffusion of specific multi-drug resistance plasmids in different environments. (c) 2005 Elsevier B.V. All rights reserved.