Target neuron prespecification in the olfactory map of Drosophila
Target neuron prespecification in the olfactory map of Drosophila
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DOI：
10.1038/35102574
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发表时间：
2001-11-08
期刊：
影响因子：
64.8
通讯作者：
Luo, LQ
中科院分区：
文献类型：
--
作者：
Jefferis, GSXE;Marin, EC;Luo, LQ
在果蝇和小鼠中，表达相同受体的嗅觉受体神经元（ORN）具有会聚的轴突投射到触角叶/嗅球中的特定肾小球靶点，在中枢神经系统的第一个嗅觉结构中创建气味图（1-3）。果蝇触角叶的投射神经元将树突发送到肾小球，并将轴突发送到更高的大脑中心（4），从而将这种气味图进一步转移到大脑中。在这里，我们使用MARCM方法（5）对投射神经元进行系统的克隆分析，使我们能够将投射神经元的谱系和出生时间与其肾小球选择相关联。我们证明，投射神经元预先指定的血统和出生顺序，形成一个突触与特定的传入ORN轴突，因此携带特定的嗅觉信息。这种预先指定可以用来固定苍蝇的嗅觉系统，使其对气味产生刻板的行为反应。发育研究使我们假设识别分子确保了ORN和投射神经元的特异性连接。这些研究还暗示了突触后神经元在确定连接特异性中精确的树突靶向作用的先前未预料到的作用。
In Drosophila and mice, olfactory receptor neurons (ORNs) expressing the same receptors have convergent axonal projections to specific glomerular targets in the antennal lobe/olfactory bulb, creating an odour map in this first olfactory structure of the central nervous system(1-3). Projection neurons of the Drosophila antennal lobe send dendrites into glomeruli and axons to higher brain centres(4), thereby transferring this odour map further into the brain. Here we use the MARCM method(5) to perform a systematic clonal analysis of projection neurons, allowing us to correlate lineage and birth time of projection neurons with their glomerular choice. We demonstrate that projection neurons are prespecified by lineage and birth order to form a synapse with specific incoming ORN axons, and therefore to carry specific olfactory information. This prespecification could be used to hardwire the fly's olfactory system, enabling stereotyped behavioural responses to odorants. Developmental studies lead us to hypothesize that recognition molecules ensure reciprocally specific connections of ORNs and projection neurons. These studies also imply a previously unanticipated role for precise dendritic targeting by postsynaptic neurons in determining connection specificity.