Hemoglobin immobilized with modified "fish-in-net" approach for the catalytic removal of aniline
Hemoglobin immobilized with modified "fish-in-net" approach for the catalytic removal of aniline
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DOI：
10.1016/j.jhazmat.2012.03.008
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发表时间：
2012-05-30
影响因子：
13.6
通讯作者：
Li, Zhengqiang
中科院分区：
文献类型：
--
作者：
Liu, Jiayin;Guan, Jingqi;Li, Zhengqiang
血液是屠宰业的废物，而其主要成分血红蛋白（Hb）是一种假过氧化物酶，能够在H2 O2存在下氧化多环芳烃（PAH）。为了将血红蛋白用于废水处理，我们采用改进的“渔网法”将血红蛋白包埋在硅基基质中。用SEM、TEM对催化剂进行了表征。BET和固态紫外-可见光谱。结果发现，血红蛋白是部分均匀分散在微球，并表现出更稳定的过氧化物酶样活性比游离血红蛋白。此外，它具有显著增加的储存稳定性以及pH稳定性。将其作为生物催化剂用于去除水中苯胺，苯胺去除率降低了65%，而在添加剂PEG的存在下，苯胺去除率降低了76%。在10次运行后没有观察到显著的活性损失。这些实验结果表明，所得产物是一种很有前途的芳烃废水处理生物催化剂。(C)2012爱思唯尔有限公司版权所有。
Blood is a waste product of the slaughter industry, while its main component hemoglobin (Hb) is a pseudo-peroxidase which is able to oxidize polycyclic aromatic hydrocarbons (PAHs) in the presence of H2O2. In order to use Hb for wastewater treatment, we encapsulated it in silica-based matrix by modified "fish-in-net" approach. The as-synthesized catalysts were characterized by SEM, TEM. BET and solid-state UV-vis spectroscopy. It was found that Hb was partially homogeneously dispersed in microspheres and showed more stable peroxidase-like activity than free Hb. Moreover, it had substantially increased storage stability as well as pH stability. It was used as biocatalyst to remove aniline in aqueous solution and gave a reduction of 65% aniline removal, while 76% in the presence of additive PEG. No significant activity loss was observed after ten runs. These experimental results suggest that the resultant product was a promising biocatalyst for aromatic wastewater treatment. (C) 2012 Elsevier B.V. All rights reserved.