Identification of an idiotypic peptide recognized by autoantibodies in human immunodeficiency virus-1-infected individuals.
Identification of an idiotypic peptide recognized by autoantibodies in human immunodeficiency virus-1-infected individuals.
复制标题
人类免疫缺陷病毒-1感染个体中自身抗体识别的独特型肽的鉴定。
DOI：
10.1172/jci118122
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Kohler,H
中科院分区：
文献类型：
--
作者：
Wang,QL;Wang,HT;Blalock,E;Muller,S;Kohler,H
HIV-1感染者体内抗HIV-1蛋白的抗体具有交叉反应独特型，由单克隆抗独特型抗体1F 7定义（5）。使用基于分子识别理论的计算机算法，确定了1F 7可变序列与人单克隆抗HIV-1抗体之间的反向亲水性区域，该区域被认为参与独特型-抗独特型接触。从人抗体的可变重链框架3-互补决定区3（FR 3-CDR 3）中的提议接触设计肽并合成。该肽被抗独特型1F 7识别，并抑制1F 7与表达1F 7独特型的人抗HIV-1抗体结合。对正常和HIV-1感染的血清的研究揭示了在感染的血清中存在与FR 3-CDR 3肽结合的抗体。针对与HIV-1感染相关的自身独特位的自身抗体的生物学相关性在调节HIV-1抗体应答的背景下进行了讨论。
Antibodies against HIV-1 proteins in HIV-1-infected individuals share a cross-reactive idiotype defined by the monoclonal antiidiotypic antibody 1F7 (5). Using a computer algorithm based on the molecular recognition theory, regions of inverse hydropathy between the variable sequence of 1F7 and human monoclonal anti-HIV-1 antibodies were identified, which are assumed to be involved in idiotype-antiidiotype contacts. A peptide was designed from the proposed contact in the variable heavy chain framework 3-complementarity determining region 3 (FR3-CDR3) of human antibodies and was synthesized. This peptide is recognized by the antiidiotype 1F7 and inhibits the binding of 1F7 to human anti-HIV-1 antibodies which express the 1F7 idiotype. A survey of normal and HIV-1-infected sera revealed the presence of antibodies in infected sera which bind to the FR3-CDR3 peptide. The biological relevance of autoantibodies against a self idiotope associated with HIV-1 infection is discussed in the context of the regulation of the antibody response to HIV-1.