Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study.
Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X-ray Scattering (SAXS) Study.
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脂质双层中碳纳米管孔蛋白的结构：原位小角 X 射线散射 (SAXS) 研究。
DOI：
10.1021/acs.nanolett.6b00466
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发表时间：
2016
期刊：
影响因子：
10.8
通讯作者：
vanBuuren,Tony
中科院分区：
文献类型：
--
作者：
Tran,IchC;Tunuguntla,RamyaH;Kim,Kyunghoon;Lee,JonathanRI;Willey,TrevorM;Weiss,ThomasM;Noy,Aleksandr;vanBuuren,Tony
碳纳米管孔蛋白（CNTPs），能够在脂质膜中形成限定孔的碳纳米管的小片段，是生物纳米电子器件的重要未来组件，因为它们可以提供生物膜通道的稳健模拟。为了控制这些CNT通道掺入到脂质双层中，重要的是要了解CNT插入到脂质双层中之前和之后的结构以及这种插入对双层结构的影响。在这里，我们采用了一种非侵入性的原位探针，小角X射线散射，研究整合的碳纳米管孔蛋白到二油酰磷脂酰胆碱双层。我们的研究结果表明，CNTPs在溶液中是稳定的单层脂质分子包裹在其外表面。我们还表明，插入到脂质双层的CNTPs的结果，在减少双层厚度的这种效果的大小增加与CNTPs的浓度。
Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. In order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. Our results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.