Single-cell analysis of cell identity in the Arabidopsis root apical meristem: insights and opportunities.
Single-cell analysis of cell identity in the Arabidopsis root apical meristem: insights and opportunities.
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DOI：
10.1093/jxb/erab228
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发表时间：
2021-05
影响因子：
6.9
通讯作者：
R. Shahan;Trevor M. Nolan;P. Benfey
中科院分区：
文献类型：
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作者：
R. Shahan;Trevor M. Nolan;P. Benfey
发育生物学中的一个基本问题是干细胞的后代如何成为分化的组织。由于其简单的组织结构和明确的细胞谱系，拟南芥的根是解决这一问题的一个容易处理的模型。特别是，根尖的分裂细胞区域，即根尖分生组织，提供了绘制干细胞生态位维持、组织构型和细胞特性获取基础的基因调控网络的机会。为了确定这些过程的分子调控因素，过去20年的研究采用了基因表达模式的全球图谱。然而，这些技术容易由于平均多种细胞类型和/或发育阶段的基因表达特征而导致信息丢失。最近开发的高通量方法在单细胞分辨率下分析基因表达已经成功地应用于植物。在这里，我们回顾了首次发表的来自拟南芥根的单细胞mRNA测序和染色质可及性数据集的见解。这些论文成功地重建了发育轨迹，以前所未有的分辨率表现出细胞身份突变，并揭示了细胞类型对环境刺激的特定反应。从拟南芥获得的实验洞察力为描述其他物种的根铺平了道路。
A fundamental question in developmental biology is how the progeny of stem cells become differentiated tissues. The Arabidopsis root is a tractable model to address this question due to its simple organization and defined cell lineages. In particular, the zone of dividing cells at the root tip, the root apical meristem, presents an opportunity to map the gene regulatory networks underlying stem cell niche maintenance, tissue patterning, and cell identity acquisition. To identify molecular regulators of these processes, studies over the last twenty years employed global profiling of gene expression patterns. However, these technologies are prone to information loss due to averaging gene expression signatures over multiple cell types and/or developmental stages. Recently developed high-throughput methods to profile gene expression at single-cell resolution have been successfully applied to plants. Here, we review insights from the first published single-cell mRNA sequencing and chromatin accessibility datasets generated from Arabidopsis roots. These papers successfully reconstruct developmental trajectories, phenotype cell identity mutants at unprecedented resolution, and reveal cell-type-specific responses to environmental stimuli. The experimental insight gained from Arabidopsis paves the way to profile roots from additional species.