The effect of pH on the toxicity of fatty acids and fatty acid amides to rainbow trout gill cells
The effect of pH on the toxicity of fatty acids and fatty acid amides to rainbow trout gill cells
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DOI：
10.1016/j.aquatox.2013.09.026
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发表时间：
2014-01-01
影响因子：
4.5
通讯作者：
Moeller, Peter D. R.
中科院分区：
文献类型：
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作者：
Bertin, Matthew J.;Voronca, Delia C.;Moeller, Peter D. R.
有害藻华 (HAB) 在短时间内使水生生物面临多种物理和化学压力。藻类毒素本身可能会因影响其生物利用度和由此产生的毒性的水化学参数而改变。本研究的目的是确定两种非生物参数（pH、无机金属盐）对脂肪酸酰胺和脂肪酸毒性的影响，脂肪酸酰胺和脂肪酸是有害藻类（包括对水生生物有毒的金藻、Prymnesium parvum）产生的两类脂质。虹鳟鱼鳃细胞被用作鱼鳃模型，并暴露于单一化合物和化合物混合物以及 pH 水平和无机金属盐浓度的变化。我们采用人工神经网络（ANN）和标准方差分析统计分析来检查和预测这些非生物参数对脂肪酸酰胺和脂肪酸毒性的影响。我们的结果表明，增加 pH 值会增加脂肪酸酰胺的毒性并抑制脂肪酸的毒性。这种现象在较低的 pH 值下会逆转。与脂肪酸酰胺和脂肪酸的单一化合物测试相比，将鳃细胞暴露于化学因子的复杂混合物中会导致毒性急剧增加。这些发现强调了物理化学因素影响藻华期间释放的化学物质毒性的潜力，并证明 pH 对脂肪酸酰胺和脂肪酸的影响存在巨大差异。由 Elsevier B.V. 出版
Harmful algal blooms (HABs) expose aquatic organisms to multiple physical and chemical stressors during an acute time period. Algal toxins themselves may be altered by water chemistry parameters affecting their bioavailability and resultant toxicity. The purpose of this study was to determine the effects of two abiotic parameters (pH, inorganic metal salts) on the toxicity of fatty acid amides and fatty acids, two classes of lipids produced by harmful algae, including the golden alga, Prymnesium parvum, that are toxic to aquatic organisms. Rainbow trout gill cells were used as a model of the fish gill and exposed to single compounds and mixtures of compounds along with variations in pH level and concentration of inorganic metal salts. We employed artificial neural networks (ANNs) and standard ANOVA statistical analysis to examine and predict the effects of these abiotic parameters on the toxicity of fatty acid amides and fatty acids. Our results demonstrate that increasing pH levels increases the toxicity of fatty acid amides and inhibits the toxicity of fatty acids. This phenomenon is reversed at lower pH levels. Exposing gill cells to complex mixtures of chemical factors resulted in dramatic increases in toxicity compared to tests of single compounds for both the fatty acid amides and fatty acids. These findings highlight the potential of physicochemical factors to affect the toxicity of chemicals released during algal blooms and demonstrate drastic differences in the effect of pH on fatty acid amides and fatty acids. Published by Elsevier B.V.