MECHANISM FOR FORMATION OF THE SECONDARY WALL THICKENING IN TRACHEARY ELEMENTS - MICROTUBULES AND MICROFIBRILS OF TRACHEARY ELEMENTS OF PISUM-SATIVUM L AND COMMELINA-COMMUNIS L AND THE EFFECTS OF AMIPROPHOSMETHYL
MECHANISM FOR FORMATION OF THE SECONDARY WALL THICKENING IN TRACHEARY ELEMENTS - MICROTUBULES AND MICROFIBRILS OF TRACHEARY ELEMENTS OF PISUM-SATIVUM L AND COMMELINA-COMMUNIS L AND THE EFFECTS OF AMIPROPHOSMETHYL
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DOI：
10.1007/bf00194060
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发表时间：
1991-01-01
期刊：
影响因子：
4.3
通讯作者：
HOGETSU, T
中科院分区：
文献类型：
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作者：
HOGETSU, T
用冰冻切片复制法和免疫荧光显微镜观察了豌豆和鸭茅的管状分子中微纤维和微管的排列。豌豆和鸭茅根的次生壁厚度分别为凹坑状和网格状。在雌蕊的凹坑增厚中，大多数MF在绕着凹坑的外围移动时保持平行排列。然而，一些基团的MFS生长到坑内，但随后在增厚的边缘终止，这表明纤维素-合成酶复合体在坑下的质膜中失活。豌豆和骨膜的TES微管位于次生增厚的下方，在壁加厚之间的区域很少检测到MTS。在MT干扰剂存在的情况下，豆根、豌豆上胚轴和鸭茅根的TES中以变形的方式沉积了专用于次生增厚的胺丙磷、纤维素和半纤维素。这表明半纤维素和纤维素的局部沉积涉及MTS。变形但不均匀的次生增厚模式仍然可见，表明MT参与了决定和维持次生增厚的规律性模式，而不是壁面沉积的空间非均质性模式。提出了二次增厚形成的工作假说。
Arrangements of microfibrils (MFs) and microtubules (MTs) were examined in tracheary elements (TEs) of Pisum sativum L. and Commelina communis L. by production of replicas of cryo-sections, and by immunofluorescence microscopy, respectively. The secondary wall thickenings of TEs of Pisum and Commelina roots have pitted and latticed patterns, respectively. Most MFs in the pitted thickening of Pisum TEs retain a parallel alignment as they pass around the periphery of pits. However, some groups of MFs grow into the pits but then terminate at the edge of the thickening, indicating that cellulose-synthase complexes are inactivated in the plasma membrane under the pit. Microtubules of TEs of both Pisum and Commelina are localized under the secondary thickening and few MTs are detected in the areas between wall thickenings. In the presence of the MT-disrupting agent, amiprophosmethyl, cellulose and hemicellulose, which is specific to secondary thickening, are deposited in deformed patterns in TEs of Pisum roots, Pisum epicotyls and Commelina roots. This indicates that the localized deposition of hemicellulose as well as cellulose involves MTs. The deformed, but heterogeneous pattern of secondary thickening is still visible, indicating that MTs are involved in determining and maintaining the regular patterns of the secondary thickening but not the spatial heterogeneous pattern of the wall deposition. A working hypothesis for the formation of the secondary thickening is proposed.