Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes
Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes
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DOI：
10.1016/s1388-2481(02)00451-4
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发表时间：
2002-10-01
影响因子：
5.4
通讯作者：
Lin, YH
中科院分区：
文献类型：
--
作者：
Musameh, M;Wang, J;Lin, YH
描述了碳纳米管（CNT）修饰的玻碳电极对NADH表现出强而稳定的电催化响应。使用单壁和多壁碳纳米管涂层观察到NADH氧化反应的过电压的显著（490 mV）降低（与普通碳电极相比），氧化开始于约100 mV。-0.05 V（相对于Ag/AgCl; pH 7.4）。此外，涂层电极的NADH安培响应非常稳定，在2 × 10（-4）和5 × 10（-3）M NADH溶液搅拌60分钟后，分别保留96%和90%的初始活性（相比之下，裸表面为20%和14%）。因此，CNT涂覆的电极允许高灵敏度、低电位、稳定的安培传感。这种能力的碳纳米管，以促进NADH电子转移反应表明了巨大的承诺，为基础的酶的安培生物传感器。(C)2002 Elsevier Science B. V.保留所有权利。
Carbon-nanotube (CNT)-modified glassy carbon electrodes exhibiting strong and stable electrocatalytic response toward NADH are described. A substantial (490 mV) decrease in the overvoltage of the NADH oxidation reaction (compared to ordinary carbon electrodes) is observed using single-wall and multi-wall carbon-nanotube coatings, with oxidation starting at ca. -0.05 V (vs. Ag/AgCl; pH 7.4). Furthermore, the NADH amperometric response of the coated electrodes is extremely stable, with 96% and 90% of the initial activity remaining after 60 min stirring of 2 x 10(-4) and 5 x 10(-3) M NADH solutions, respectively (compared to 20% and 14% at the bare surface). The CNT-coated electrodes thus allow highly sensitive, low-potential, stable amperometric sensing. Such ability of carbon nanotubes to promote the NADH electron-transfer reaction suggests great promise for dehydrogenase-based amperometric biosensors. (C) 2002 Elsevier Science B.V. All rights reserved.