The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice
The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice
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类AGL6基因OsMADS6调节水稻花器官和分生组织特性
DOI：
10.1038/cr.2009.143
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发表时间：
2010-03-01
期刊：
影响因子：
44.1
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Li, Haifeng;Liang, Wanqi;Zhang, Dabing
AGAMOUS-LIKE6 (AGL6) MADS-box基因是古老的，在裸子植物和被子植物中广泛分布，但其功能仍不清楚。在这里，我们展示了agl6样基因OsMADS6在水稻（Oryza sativa L.）花器官和分生组织特性中的生物学作用。OsMADS6在花分生组织早期强烈表达。随后，OsMADS6转录本主要在胚珠基部、胚珠小叶、心皮和珠被以及花托中检测到。与野生型植物相比，osmads6突变体表现出古形体特征的改变、颖片样或花叶器官的增加、心皮发育异常和花分生组织确定性的丧失。引人注目的是，SEPALLATA （SEP）样基因OsMADS1 （LHS1）的突变加剧了OsMADS1花的缺陷，OsMADS1 -z OsMADS1 -1花的第2轮和第3轮未观察到内部花器官或颖片样结构。此外，osmads1-z osmads1- 1双突变体产生了严重不确定的花分生组织。因此，我们的发现表明，古老的OsMADS6基因能够通过与OsMADS1一起决定单子叶作物水稻的花器官和分生组织特征来指定“花状态”。
Although AGAMOUS-LIKE6 (AGL6) MADS-box genes are ancient with wide distributions in gymnosperms and angiosperms, their functions remain poorly understood. Here, we show the biological role of the AGL6-like gene, OsMADS6, in specifying floral organ and meristem identities in rice (Oryza sativa L.). OsMADS6 was strongly expressed in the floral meristem at early stages. Subsequently, OsMADS6 transcripts were mainly detectable in paleas, lodicules, carpels and the integument of ovule, as well as in the receptacle. Compared to wild type plants, osmads6 mutants displayed altered palea identity, extra glume-like or mosaic organs, abnormal carpel development and loss of floral meristem determinacy. Strikingly, mutation of a SEPALLATA (SEP)-like gene, OsMADS1 (LHS1), enhanced the defect of osmads6 flowers, and no inner floral organs or glume-like structures were observed in whorls 2 and 3 of osmads1-z osmads6-1 flowers. Furthermore, the osmads1-z osmads6-1 double mutants developed severely indeterminate floral meristems. Our finding, therefore, suggests that the ancient OsMADS6 gene is able to specify “floral state” by determining floral organ and meristem identities in monocot crop rice together with OsMADS1.