Novel isolation of phytochemical compositions by phase transition extraction with acetonitrile
Novel isolation of phytochemical compositions by phase transition extraction with acetonitrile
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通过乙腈相变萃取分离植物化学成分的新方法
DOI：
10.1002/jssc.201000658
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发表时间：
2011-02-01
影响因子：
3.1
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Liu, Guozhu;Yang, Huawu;Yao, Shouzhuo
基于通过添加疏水性溶剂可以将亲水性乙腈与水分离的现象，开发了一种用于分离植物化学成分的相变萃取（PTE）方法。通过对三种重要中药植物中的成分进行完整分组来评估所提出方法的分离效率。对黄芩PTE的进一步研究发现，有机相中的成分均为黄酮苷元，而水相中的成分均为黄酮苷，揭示了所提出的PTE工艺的高结构选择性的特性。研究了使用不同改性剂对分离效率的影响。使用非氧化疏水溶剂获得的结果是完美的，而使用无机盐获得的结果是可以接受的，但是使用氧化疏水溶剂获得的结果较差。随后的 GC 分析表明，使用不同类型改性剂的 PTE 工艺中两个分离相之间的溶剂组成差异是导致获得不同分离结果的原因。最后，证明该方法对乙腈浓度和改性剂添加量均不敏感。
Based on the phenomenon that hydrophilic acetonitrile can be separated from water by adding a hydrophobic solvent, a phase transition extraction (PTE) method for isolation of phytochemical compositions was developed. The isolation efficiency of the proposed method was assessed in complete grouping of the constituents in three important traditional Chinese medicine plants. Further study of the PTE for Scutellaria bacalensis Georgi found that the constituents in the organic phase are all flavonoid aglycones while the ones in the aqueous phase are all flavonoid glycosides, revealing the property of high structure selectivity for the proposed PTE process. The effect of using different modifiers on the isolation efficiency was investigated. The results obtained by using non-oxygenated hydrophobic solvents are perfect while those by inorganic salts are acceptable, but those by oxygenated hydrophobic solvents are bad. Subsequent GC analysis revealed that the solvent composition difference between the two separated phases among PTE processes using different types of modifier is responsible for the different isolation results obtained. Finally, the proposed method was proved to be insensitive to both the acetonitrile concentration and the added amount of modifier.