Functional overlap of the Arabidopsis leaf and root microbiota
Functional overlap of the Arabidopsis leaf and root microbiota
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DOI：
10.1038/nature16192
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发表时间：
2015-12-17
期刊：
影响因子：
64.8
通讯作者：
Schulze-Lefert, Paul
中科院分区：
文献类型：
--
作者：
Bai, Yang;Mueller, Daniel B.;Schulze-Lefert, Paul
健康植物的根和叶宿主分类学结构的细菌集合体，并且这些社区的成员有助于植物生长和健康。我们建立了拟南芥叶和根来源的微生物群培养物集合，代表了大多数通过培养物独立的群落测序可重复检测的细菌物种。我们发现叶和根微生物之间存在广泛的分类重叠。400株分离株的基因组草图显示，叶和根来源的细菌之间的基因组编码的功能能力有很大的重叠，在单个功能类别的水平上几乎没有显着差异。使用定义的细菌群落和gnotobiotic拟南芥植物系统，我们表明，分离物形成类似于其同源宿主器官上的天然微生物群的组件，但也能够异位叶或根定殖。虽然这提高了根和叶微生物群成员之间相互迁移的可能性，但基因组信息和克隆化实验也为微生物群专门化到各自的生态位提供了证据。
Roots and leaves of healthy plants host taxonomically structured bacterial assemblies, and members of these communities contribute to plant growth and health. We established Arabidopsis leaf-and root-derived microbiota culture collections representing the majority of bacterial species that are reproducibly detectable by culture-independent community sequencing. We found an extensive taxonomic overlap between the leaf and root microbiota. Genome drafts of 400 isolates revealed a large overlap of genome-encoded functional capabilities between leaf-and root-derived bacteria with few significant differences at the level of individual functional categories. Using defined bacterial communities and a gnotobiotic Arabidopsis plant system we show that the isolates form assemblies resembling natural microbiota on their cognate host organs, but are also capable of ectopic leaf or root colonization. While this raises the possibility of reciprocal relocation between root and leaf microbiota members, genome information and recolonization experiments also provide evidence for microbiota specialization to their respective niche.