Biological and genetic characterization of Rickettsia sibirica strains isolated in the endemic area of the North Asian tick typhus
Biological and genetic characterization of Rickettsia sibirica strains isolated in the endemic area of the North Asian tick typhus
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DOI：
10.4269/ajtmh.1996.55.685
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Balayeva, NM;Eremeeva, ME;Raoult, D
采用聚合酶链反应扩增基因片段的限制性片段长度多态性（RFLP）分析方法，对24株斑点热群立克次体分离株进行了血清学特征分析。这些菌株于1946年至1991年在俄罗斯从人类和不同种属的硬蜱中获得。利用来自于红曲霉的属特异性引物对扩增的DNA产物进行RFLP分析。prowazekii柠檬酸合成酶基因和两对来自R.立克次氏体190-kD和120-kD表面蛋白抗原基因，随后是Alu I、Pst I和Rsa I限制性内切酶双酶切。虽然所检测的菌株之间的生物学特性存在一定的差异，但只有一个R。利用这些分子鉴定工具发现sibirica基因型。
Restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction-amplified gene fragments was used to characterize 24 isolates of spotted fever group rickettsiae previously identified as Rickettsia sibirica from their serologic properties. These strains were obtained in Russia between 1946 and 1991 from humans and different species of Ixodid ticks. The RFLP analysis was performed using amplified DNA products obtained with a genus-specific primer pair derived from the R. prowazekii citrate synthase gene and two group-specific primer pairs from the R. rickettsii 190-kD and 120-kD surface protein antigen genes followed by Alu I, Pst I, and Rsa I restriction endonuclease digestions. Although some differences were detected in biological characteristics among the examined strains, only a single R. sibirica genotype was found with these molecular tools of identification.