A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis
A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis
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DOI：
10.2217/fmb-2016-0023
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发表时间：
2016-10-01
影响因子：
3.1
通讯作者：
Zhang, Yanmei
中科院分区：
文献类型：
--
作者：
Hu, Binjie;Zhao, Fuju;Zhang, Yanmei
目的：我们建立了一个高通量多重基因检测系统（HMGS），用于鉴定幽门螺杆菌，同时进行毒力和耐药性分析。材料与方法：经132 H.通过20基因位点HMGS、测序和E-test筛选来自胃活检的pylori培养物。结果：HMGS对H.幽门螺杆菌鉴定HMGS与测序结果的一致率平均为94.5%（毒力基因）和97.3%（抗性基因）。观察到的对四种主流抗生素的耐药率很高，但阿莫西林除外。有5个基因的毒力基因型和特定胃肠道疾病的风险之间存在显著关联。消化性溃疡患者甲硝唑耐药率明显增高。结论：HMGS是治疗H.幽门螺杆菌鉴定及毒力和耐药性分析。
Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance. Materials & methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test. Results: HMGS was highly sensitive and specific for H. pylori identification. Concordance rate between HMGS and sequencing averaged 94.5% (virulence genes) and 97.3% (resistance genes). Observed resistance rates to four mainstream antibiotics were high, except for amoxicillin. Significant association between virulence genotype and risks for specific gastrointestinal diseases was found for five genes. Metronidazole resistance in peptic ulcer patients was significantly higher. Conclusion: HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.