An immune responsive factor D-like serine proteinase homologue identified from the American dog tick, Dermacentor variabilis.
An immune responsive factor D-like serine proteinase homologue identified from the American dog tick, Dermacentor variabilis.
复制标题
从美国狗蜱 (Dermacentor variabilis) 中鉴定出一种免疫反应因子 D 样丝氨酸蛋白酶同源物。
DOI：
10.1111/j.1365-2583.2004.00455.x
复制
发表时间：
2004
影响因子：
2.6
通讯作者：
Azad,AF
中科院分区：
文献类型：
--
作者：
Simser,JA;Mulenga,A;Macaluso,KR;Azad,AF
从蜱血细胞中鉴定了编码片段结构域丝氨酸蛋白酶同源物的可变性ADermacentor variabiliscDNA，其中甘氨酸取代了催化丝氨酸。变异杜氏菌产物与中国鲎血细胞抗菌因子D最相似，并与节肢动物的许多免疫应答基因产物（包括昆虫酚氧化酶原激活辅因子）具有显著的同源性。北方印迹分析证实，蜱丝氨酸蛋白酶同源物在血细胞中的表达水平最高，在卵巢和唾液腺中的表达水平较低，而在整个蜱中的稳态表达水平在进食与未进食的成虫或卵中略高。用大肠杆菌注射法对成年大鼠进行攻击，结果表明，在时间上，转录本的丰度比未感染的对照组显著增加。此外，从一个Dermacentor andersonicell细胞系中克隆了变异革蜱clip-结构域分子的一个明显的直系同源物，并检测了其表达，表明跨物种保守性。
ADermacentor variabiliscDNA encoding a clip‐domain serine proteinase homologue with glycine replacing the catalytic serine was identified from tick haemocytes. TheD. variabilisproduct was most similar toTachypleus tridentatushaemocyte antimicrobial factor D and shared significant homologies with a number of immune‐responsive gene products of arthropods, including insect prophenoloxidase‐activating cofactors. Northern blotting analyses confirmed that the tick serine proteinase homologue expression levels were highest in haemocytes, and to lesser degrees in ovaries and then salivary glands whereas steady‐state levels of expression in whole ticks were found to be slightly higher in fed versus unfed adults or eggs. Challenge of fed adults byEscherichia coliinjection demonstrated that transcript abundance was significantly increased above those of naive controls in a temporal fashion. Additionally, an apparent orthologue of theD. variabilisclip‐domain molecule was cloned, and expression detected, from aDermacentor andersonicell line indicating cross species conservation.