Genetic and Functional Analyses of Cutibacterium Acnes Isolates Reveal the Association of a Linear Plasmid with Skin Inflammation.
Genetic and Functional Analyses of Cutibacterium Acnes Isolates Reveal the Association of a Linear Plasmid with Skin Inflammation.
复制标题
痤疮皮肤杆菌分离株的遗传和功能分析揭示了线性质粒与皮肤炎症的关联。
DOI：
10.1016/j.jid.2023.05.029
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Gallo,RichardL
中科院分区：
文献类型：
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作者：
O'Neill,AlanM;Cavagnero,KellenJ;Seidman,JasonS;Zaramela,Livia;Chen,Yang;Li,Fengwu;Nakatsuji,Teruaki;Cheng,JoyceY;Tong,YunL;Do,TranH;Brinton,SamanthaL;Hata,TissaR;Modlin,RobertL;Gallo,RichardL
Cutibacterium acnesis a commensal bacterium on the skin that is generally well-tolerated, but different strain types have been hypothesized to contribute to the disease acne vulgaris. To understand how some strain types might contribute to skin inflammation, we generated a repository ofC. acnesisolates from skin swabs of healthy subjects and subjects with acne and assessed their strain-level identity and capacity to stimulate cytokine release. Phylotype II K-type strains were more frequent on healthy and nonlesional skin of subjects with acne than those isolated from lesions. Phylotype IA-1 C-type strains were increased on lesional skin compared with those on healthy skin. The capacity to induce cytokines from cultured monocyte-derived dendritic cells was opposite to this action on sebocytes and keratinocytes and did not correlate with the strain types associated with the disease. Whole-genome sequencing revealed a linear plasmid in high-inflammatory isolates within similar strain types that had different proinflammatory responses. Single-cell RNA sequencing of mouse skin after intradermal injection showed that strains containing this plasmid induced a higher inflammatory response in dermal fibroblasts. These findings revealed thatC. acnesstrain type is insufficient to predict inflammation and that carriage of a plasmid could contribute to disease.
