Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus
Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus
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DOI：
10.1128/jvi.77.18.10119-10124.2003
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发表时间：
2003-09-01
影响因子：
5.4
通讯作者：
Sonigo, P
中科院分区：
文献类型：
--
作者：
Brussel, A;Sonigo, P
开发了一种新的基于Alu-long terminal repeat （LTR）的实时巢式pcr方法，以准确和高灵敏度（50,000个细胞当量中的6个原病毒）定量感染细胞中的综合人类免疫缺陷病毒I型（HIV-1） DNA。对总HIV-1 DNA和两个ltr HIV-1 DNA环的平行测定允许在单轮病毒复制中监测不同HIV-1 DNA物种的合成和命运。
A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type I (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.