Compositional Control of Phase-Separated Cellular Bodies.
Compositional Control of Phase-Separated Cellular Bodies.
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DOI：
10.1016/j.cell.2016.06.010
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发表时间：
2016-07-28
期刊：
影响因子：
64.5
通讯作者：
Rosen MK
中科院分区：
文献类型：
--
作者：
Banani SF;Rice AM;Peeples WB;Lin Y;Jain S;Parker R;Rosen MK
细胞体如P体和PML核体（PML NB）似乎是由蛋白质和RNA分子之间的多价相互作用组织的相分离液体。虽然各种细胞体的许多组成部分是已知的，但缺乏定义身体组成的一般原则。我们使用几种工程化的多价蛋白质和RNA对细胞体进行建模。在体外和细胞中，这些支架分子形成相分离的液体，浓缩低价客户蛋白。客户端根据支架的比例进行不同的分区，在相图对角线上有一个急剧的开关。组成可以通过支架浓度或化合价的变化快速切换。天然PML NB和P体显示类似的分配行为，表明它们的组成如何分别由PML SUMO化水平或细胞mRNA浓度控制。这些数据提出了一个概念框架，考虑通过异型多价相互作用组装的细胞体的组成和控制。
Cellular bodies such as P bodies and PML nuclear bodies (PML NBs) appear to be phase-separated liquids organized by multivalent interactions among proteins and RNA molecules. Although many components of various cellular bodies are known, general principles that define body composition are lacking. We modeled cellular bodies using several engineered multivalent proteins and RNA. In vitro and in cells, these scaffold molecules form phase-separated liquids that concentrate low valency client proteins. Clients partition differently depending on the ratio of scaffolds, with a sharp switch across the phase diagram diagonal. Composition can switch rapidly through changes in scaffold concentration or valency. Natural PML NBs and P bodies show analogous partitioning behavior, suggesting how their compositions could be controlled by levels of PML SUMOylation or cellular mRNA concentration, respectively. The data suggest a conceptual framework for considering the composition and control thereof of cellular bodies assembled through heterotypic multivalent interactions.