RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa.
RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa.
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DOI：
10.1530/rep-09-0015
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发表时间：
2009-06
期刊：
影响因子：
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通讯作者：
Sarge KD
中科院分区：
文献类型：
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作者：
Wilkerson DC;Sarge KD
Hspa1b（Hsp70.1）基因是受精后表达的第一个基因之一，在没有应激的情况下，在小合子基因组激活期间表达。早在胚胎发生的单细胞阶段，这种表达就可以在雄性原核中发生。HSPA1B在应激期间对胚胎活力的重要性得到了研究的支持，该研究表明，这种蛋白质的消耗导致胚胎发育到胚细胞阶段的显着减少。最近，我们开始解决的机制，负责允许Hspa1b在小ZGA的表达，并发现，HSF1和HSF2结合Hspa1b启动子在精子发生后期。在这份报告中，我们已经扩展了这些研究，使用蛋白质印迹和染色质免疫沉淀试验，发现RNA聚合酶II是存在于附睾精子和绑定到Hspa1b启动子。这些结果，除了我们以前的结果，支持一个模型，其中的HSF1，HSF2，Sp1，和RNA聚合酶II的Hspa1b的启动子的结合将允许快速形成一个转录能力的状态在次要ZGA，从而允许Hspa1b的表达。
The Hspa1b (Hsp70.1) gene is one of the first genes expressed after fertilization, with expression occurring during the minor zygotic genome activation in the absence of stress. This expression can take place in the male pronucleus as early as the one-cell stage of embryogenesis. The importance of HSPA1B for embryonic viability during times of stress is supported by studies showing that depletion of this protein results in a significant reduction in embryos developing to the blastocyte stage. Recently we began addressing the mechanism responsible for allowing expression of Hspa1b during the minor ZGA and found that HSF1 and HSF2 bind the Hspa1b promoter during late spermatogenesis. In this report, we have extended those studies using western blots and chromatin immunoprecipitation assays and found that RNA Polymerase II is present in epididymal spermatozoa and bound to the Hspa1b promoter. These present results, in addition to our previous results, support a model in which the binding of HSF1, HSF2, Sp1, and RNA Polymerase II to the promoter of Hspa1b would allow the rapid formation of a transcription-competent state during the minor ZGA, thereby allowing Hspa1b expression.