Purification and Characterization of a Novel Sex Pheromone that Induces the Release of Another Sex Pheromone during Sexual Reproduction of the Heterothallic Closterium peracerosum-strigosum-littorale Complex
Purification and Characterization of a Novel Sex Pheromone that Induces the Release of Another Sex Pheromone during Sexual Reproduction of the Heterothallic Closterium peracerosum-strigosum-littorale Complex
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一种新型性信息素的纯化和表征，该性信息素在异菌新月藻-strigosum-littorale复合体的有性繁殖过程中诱导另一种性信息素的释放
DOI：
10.1093/oxfordjournals.pcp.a078747
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发表时间：
1995
影响因子：
4.9
通讯作者：
H. Sekimoto
中科院分区：
文献类型：
--
作者：
T. Nojiri;T. Fujii;H. Sekimoto
通过测定新月藻（Closterium）交配型负细胞释放的性信息素PR-IP诱导剂对交配型正细胞释放的原生质体释放诱导蛋白（PR-IP）的生物学效应，得到了一种纯化的性信息素PR-IP诱导剂。经SDS-PAGE和质谱分析，纯化的PR-IP诱导剂的表观分子量分别为22 kDa和18.7 kDa。用高碘酸-希夫试剂染色表明PR-IP诱导剂包含聚糖链。从剂量-反应曲线的分析来看，PR-IP诱导剂似乎能够在相当宽的浓度范围内发挥其活性（1 × 10−10−3 × 10− 7 M）。看来PR-IP诱导剂是一种新的糖蛋白类信息素，PR-IP也是如此，并且它在theClosterium peracerosum-strigosum-littoralcomplex有性生殖的最早阶段发挥作用。
A sex pheromone, PR-IP Inducer, which is released from mating-type minus cells ofClosterium, was purified by monitoring its biological effect on the induction of the release of protoplast-release-inducing protein (PR-IP) from mating-type plus cells. The purified PR-IP Inducer had an apparent molecular mass of 22 kDa and of 18.7 kDa as determined by SDS-PAGE and mass-spectrometric analysis, respectively. Staining with periodic acid-Schiff reagent indicated that PR-IP Inducer included a glycan chain. From the analysis of a dose-response curve, it seemed that PR-IP Inducer was able to exert its activity over quite wide range of concentrations (1 × 10−10−3 × 10−7M). It appears that PR-IP Inducer is a novel glycoproteinaceous pheromone, as is PR-IP, and that it exerts its effect at the earliest stages of the sexual reproduction of theClosterium peracerosum-strigosum-littoralecomplex.