A quantitative J-correlation experiment for the accurate measurement of one-bond amide N-15-H-1 couplings in proteins
A quantitative J-correlation experiment for the accurate measurement of one-bond amide N-15-H-1 couplings in proteins
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DOI：
10.1006/jmrb.1996.0138
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Prestegard, JH
中科院分区：
文献类型：
--
作者：
Tolman, JR;Prestegard, JH
用两种不同的实验方法对（1）氰高铁肌红蛋白中约40%的酰胺位的J（NH）偶合进行了非常精确的测量。第一种方法是以前描述过的基于频率的方法，其中偶合在频域谱中被观察为分裂。第二种方法是一种新的方法，沿着定量J-相关谱线，其中耦合被编码在共振强度中。从这两种实验技术获得的测量结果符合高精度（s.d.）。的0.17 Hz）和残余偏差似乎是很大程度上随机的，新的方法提供了大量的时间节省时，谐振广泛分散在频域中，并可以提供在这些情况下，提高精度。(C)出版社：Academic Press，Inc.
Very precise measurements of (1)J(NH) couplings have been made for approximately 40% of the amide sites in cyanometmyoglobin using two different experimental approaches, The first approach is a previously described frequency-based method in which the couplings are observed as splittings in the frequency-domain spectrum, The second is a new approach, along the lines of quantitative J-correlation spectroscopy, in which the coupling is encoded in the resonance intensity. Measurements obtained from the two experimental techniques are in agreement to a high degree of precision (s.d. of 0.17 Hz) and residual deviations appear to be largely random, The new method offers substantial time savings when resonances are widely dispersed in the frequency domain and may offer improved precision in these instances. (C) 1996 Academic Press, Inc.