Blood cannabinoid molar metabolite ratios are superior to blood THC as an indicator of recent cannabis smoking.
Blood cannabinoid molar metabolite ratios are superior to blood THC as an indicator of recent cannabis smoking.
复制标题
作为近期吸食大麻的指标，血液大麻素摩尔代谢物比率优于血液 THC。
DOI：
10.1080/15563650.2023.2214697
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Brooks-Russell,Ashley
中科院分区：
文献类型：
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作者：
Kosnett,MichaelJ;Ma,Ming;Dooley,Gregory;Wang,GeorgeSam;Friedman,Kyle;Brown,Timothy;Henthorn,ThomasK;Brooks-Russell,Ashley
大麻的使用在运输和工作场所事件中越来越受到关注。由于Δ9-四氢大麻酚在急性精神活性作用消退后可检测到，因此其作为近期使用或潜在损害的指标具有局限性。我们测量了全血Δ9-四氢大麻酚及其代谢物11-羟基-Δ9-四氢大麻酚和11-去甲-9-羧基-Δ9-四氢大麻酚，在24名偶尔和32名每日吸食大麻者中，在基线和开始间隔15分钟吸食大麻后30分钟，通过液相色谱串联质谱法测定四氢大麻酚。我们计算了两种血液大麻素摩尔代谢物比率：1）[Δ9-四氢大麻酚]与[11-去甲-9-羧基-Δ9-四氢大麻酚]和2）（[Δ9-四氢大麻酚] + [11-羟基-Δ9-四氢大麻酚]）与[11-去甲-9-羧基-Δ9-四氢大麻酚]。我们将这些与血液[Δ9-tetrahydrocannabinol]单独作为近期大麻吸烟的指标进行了比较。相比之下，Δ9-四氢大麻酚的5.3微克/升的临界点产生88%的特异性，73%的灵敏度，和80%accuracy.ConclusionsIn偶尔和日常用户，血液大麻素摩尔代谢物的比率是上级全血Δ9-四氢大麻酚作为近期大麻吸烟的指标。我们建议在法医和安全性调查中测量和报告Δ9-四氢大麻酚、11-羟基-Δ9-四氢大麻酚和11-去甲-9-羧基-Δ9-四氢大麻酚及其摩尔代谢物比率。
IntroductionCannabis use is a growing concern in transportation and workplace incidents. Because Δ9-tetrahydrocannabinol is detectable after acute psychoactive effects have resolved, it has limitations as an indicator of recent usage or potential impairment.MethodsIn an observational study of driving and psychomotor performance, we measured whole blood Δ9-tetrahydrocannabinol plus its metabolites 11-hydroxy-Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol by liquid chromatography with tandem mass spectrometry at baseline and 30 min after starting a 15-minute interval of smoking cannabis in 24 occasional and 32 daily cannabis smokers. We calculated two blood cannabinoid molar metabolite ratios: 1) [Δ9-tetrahydrocannabinol] to [11-nor-9-carboxy-Δ9-tetrahydrocannabinol] and 2) ([Δ9-tetrahydrocannabinol] + [11-hydroxy-Δ9-tetrahydrocannabinol]) to [11-nor-9-carboxy-Δ9-tetrahydrocannabinol]. We compared these to blood [Δ9-tetrahydrocannabinol] alone as indicators of recent cannabis smoking.ResultsMedian Δ9-tetrahydrocannabinol concentrations increased from 0 (P > 0.38). By comparison, a cut-point for Δ9-tetrahydrocannabinol of 5.3 µg/L yielded 88% specificity, 73% sensitivity, and 80% accuracy.ConclusionsIn occasional and daily users, the blood cannabinoid molar metabolite ratios were superior to whole blood Δ9-tetrahydrocannabinol as indicators of recent cannabis smoking. We recommend measurement and reporting of Δ9-tetrahydrocannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol, and their molar metabolite ratios in forensic and safety investigations.