Anti-cachectic effect of ghrelin in nude mice bearing human melanoma cells
Anti-cachectic effect of ghrelin in nude mice bearing human melanoma cells
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DOI：
10.1016/s0006-291x(02)03028-0
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发表时间：
2003-02-07
影响因子：
3.1
通讯作者：
Nakazato, M
中科院分区：
文献类型：
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作者：
Hanada, T;Toshinai, K;Nakazato, M
Ghrelin是一种新的脑肠肽，刺激食物摄入和体重增加。我们研究了Ghrelin对癌症恶病质小鼠模型合成代谢的影响。将人黑色素瘤细胞系SEKI接种到裸鼠中，以检查ghrelin对食物摄入和体重的影响。每天两次（6 nmol/小鼠/天）腹膜内给予生长素释放肽6天，抑制了SEKI接种小鼠的体重减轻，并增加了载体处理的裸鼠的体重增加速率。Ghrelin给药也增加了SEKI和溶媒治疗小鼠的食物摄入量。无论是白色脂肪组织的重量和血浆瘦素浓度降低肿瘤接种的小鼠与车辆治疗的小鼠相比，这些因素增加胃饥饿素管理。在接种肿瘤的小鼠中，胃中的ghrelin肽和mRNA的水平均上调。生长素释放肽的合成代谢作用有效地逆转了携带SEKI人黑色素瘤的小鼠的恶病质。因此，Ghrelin可能具有改善癌症恶病质的治疗能力。(C)2003 Elsevier Science（美国）。All rights reserved.
Ghrelin is a novel brain-gut peptide that stimulates food intake and body weight gain. We studied the anabolic effect of ghrelin in cancer cachexia mouse model. SEKI, a human melanoma cell line, was inoculated into nude mice to examine the effects of ghrelin on food intake and body weight. The intraperitoneal administration of ghrelin twice a day (6 nmol/mice/day) for 6 days suppressed weight loss in SEKI-inoculated mice and increased the rate of weight gain in vehicle-treated nude mice. Ghrelin administration also increased food intake in both SEKI- and vehicle-treated mice. Both the weight of white adipose tissue and the plasma leptin concentration were reduced in tumor-inoculated mice compared with vehicle-treated mice; these factors increased following ghrelin administration. The levels of both ghrelin peptide and mRNA in the stomach were upregulated in tumor-inoculated mice. The anabolic effect of ghrelin efficiently reverses the cachexia in mice bearing SEKI human melanoma. Ghrelin therefore may have a therapeutic ability to ameliorate cancer cachexia. (C) 2003 Elsevier Science (USA). All rights reserved.