Metabonomic analysis of the therapeutic effect of Potentilla discolor in the treatment of type 2 diabetes mellitus
Metabonomic analysis of the therapeutic effect of Potentilla discolor in the treatment of type 2 diabetes mellitus
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委陵菜治疗2型糖尿病的代谢组学分析
DOI：
10.1039/c4mb00278d
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Yang, Jie
中科院分区：
文献类型：
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作者：
Li, Yi;Li, Jing-jing;Yang, Jie
随着肥胖的流行，2 型糖尿病 (T2DM) 在全球范围内呈上升趋势。委陵菜是中药治疗高血糖、高血脂最重要的药材之一。在这项工作中，采用基于UPLC-Q-TOF与多变量数据分析相结合的血浆代谢组学方法来研究变色紫草提取物（EPD）和变色紫草的主要生物活性化合物科罗索酸（CA）的治疗效果。雄性C57BL/6小鼠用高脂饮食（HFD喂养组）喂养8周，然后用EPD（EPD治疗组）或CA（CA治疗组）治疗另外8周。实验结束后，收集血浆样品并通过超高效液相色谱/四极杆飞行时间质谱（UPLC-Q-TOF）进行分析。建立主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）模型来寻找T2DM的生物标志物并研究EPD和CA的治疗效果。分布在多种代谢途径中的 26 种代谢物被鉴定为 T2DM 的潜在生物标志物。研究发现EPD和CA可以通过调节紊乱的代谢途径来逆转T2DM的病理过程。代谢组学结果不仅有利于评价中药的治疗效果，而且有利于阐明潜在的分子机制。
Type 2 diabetes mellitus (T2DM) is increased worldwide in parallel with the obesity epidemic. Potentilla discolor is one of the most important crude materials in Traditional Chinese medicine (TCM) for therapy of hyperglycemia and hyperlipidemia. In this work, a plasma metabonomic approach based on the combination of UPLC-Q-TOF with multivariate data analysis was applied to investigate the therapeutic effects of the extract of P. discolor (EPD) and corosolic acid (CA), the main bioactive compounds of P. discolor. Male C57BL/6 mice were fed with high-fat diet (HFD-fed group) for 8 weeks and then treated with EPD (EPD-treated group) or CA (CA-treated group) for another 8 weeks. After the experimental period, samples of plasma were collected and analyzed by ultra-performance liquid chromatography/quadrupole time of flight mass spectrometry (UPLC-Q-TOF). The principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models were built to find biomarkers of T2DM and investigate the therapeutic effects of EPD and CA. 26 metabolites, which are distributed in several metabolic pathways, were identified as potential biomarkers of T2DM. It was found that EPD and CA could reverse the pathological process of T2DM through regulating the disturbed pathway of metabolism. The metabonomic results are beneficial not only for the evaluation of the therapeutic effect of TCM but also for the elucidation of the underlying molecular mechanism.